A new Schizosaccharomyces pombe chronological lifespan assay reveals that caloric restriction promotes efficient cell cycle exit and extends longevity.
A new Schizosaccharomyces pombe chronological lifespan assay reveals that caloric restriction promotes efficient cell cycle exit and extends longevity.
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DOI：
10.1016/j.exger.2009.04.004
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发表时间：
2009-08
影响因子：
3.9
通讯作者：
Runge, Kurt W.
中科院分区：
文献类型：
--
作者：
Chen, Bo-Ruei;Runge, Kurt W.
我们描述了一个新的时间顺序寿命（CLS）测定酵母裂糖酵母pombe。酵母CLS检测监测培养物中细胞活力随着时间的推移而丧失，这种新的检测显示，在培养物中所有细胞死亡之前，细胞活力持续下降而无法检测到再生。因此，存活曲线不会因实验中生长的突变体的产生而改变，并且可以监测菌株的整个寿命，直到活细胞数量减少超过106倍。CLS分析总结了过度营养导致的寿命缩短、热量限制导致的寿命延长、热量限制细胞的抗逆性增强以及AKT激酶对寿命的控制等进化保守特征。这两个S. pombe AKT激酶同源基因都能调节CLS：在营养过剩条件下sck1+延长寿命的缺失，在正常和营养过剩条件下sck2+延长寿命的缺失，这两个基因的缺失表明sck1+和sck2+控制着不同的寿命途径。寿命最长的S. pombe细胞表现出最有效的细胞周期退出，表明热量限制将这两个过程联系起来。这种新的S. pombe CLS检测方法将为衰老研究提供有价值的工具。
We describe a new chronological lifespan (CLS) assay for the yeast Schizosaccharomyces pombe. Yeast CLS assays monitor the loss of cell viability in a culture over time, and this new assay shows a continuous decline in viability without detectable regrowth until all cells in the culture are dead. Thus, the survival curve is not altered by the generation of mutants that can grow during the experiments, and one can monitor the entire lifespan of a strain until the number of viable cells has decreased over 106-fold. This CLS assay recapitulates the evolutionarily conserved features of lifespan shortening by over nutrition, lifespan extension by caloric restriction, increased stress resistance of calorically restricted cells and lifespan control by the AKT kinases. Both S. pombe AKT kinase orthologs regulate CLS: loss of sck1+ extended lifespan in over nutrition conditions, loss of sck2+ extended lifespan under both normal and over nutrition conditions, and loss of both genes showed that sck1+ and sck2+ control different longevity pathways. The longest-lived S. pombe cells showed the most efficient cell cycle exit, demonstrating that caloric restriction links these two processes. This new S. pombe CLS assay will provide a valuable tool for aging research.
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影响因子：
4.2
作者：
Lee HG;Casadesus G;Zhu X;Castellani RJ;McShea A;Perry G;Petersen RB;Bajic V;Smith MA
通讯作者：
Smith MA
影响因子：
10.5
作者：
Hamilton, B;Doug, YQ;Lee, SS
通讯作者：
Lee, SS
影响因子：
2.7
作者：
Kurzhals, Rebeccah L.;Tie, Feng;Harte, Peter J.
通讯作者：
Harte, Peter J.
DOI：
10.1083/jcb.200404002
发表时间：
2004-09-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fabrizio P;Battistella L;Vardavas R;Gattazzo C;Liou LL;Diaspro A;Dossen JW;Gralla EB;Longo VD
通讯作者：
Longo VD
影响因子：
56.9
作者：
Fabrizio, P;Pozza, F;Longo, VD
通讯作者：
Longo, VD