Host-to-pathogen gene transfer facilitated infection of insects by a pathogenic fungus.
Host-to-pathogen gene transfer facilitated infection of insects by a pathogenic fungus.
复制标题
宿主到病原体的基因转移促进病原真菌对昆虫的感染
DOI：
10.1371/journal.ppat.1004009
复制
发表时间：
2014-04
期刊：
影响因子：
6.7
通讯作者：
Fang W
中科院分区：
文献类型：
--
作者：
Zhao H;Xu C;Lu HL;Chen X;St Leger RJ;Fang W
罗伯茨绿僵菌是一种植物根系定殖真菌，也是一种昆虫病原菌。其昆虫致病性是在进化过程中从植物内生菌祖先获得的特征。这种转变提供了一个新的视角，对宿主转换和毒力重要的新功能机制的演变。从一个随机的T-DNA插入文库中，我们获得了一个致病性缺陷的突变体，导致从一个甾醇载体基因（Mr-npc 2a）的破坏。系统发育分析表明，绿僵菌通过水平基因转移（HGT）从昆虫身上获得了Mr-npc 2a。Mr-NPC 2a与胆固醇（一种动物甾醇）结合，而不是与真菌甾醇麦角甾醇结合，表明它保留了昆虫NPC 2蛋白的特异性。Mr-NPC 2a是一种细胞内蛋白，仅在活昆虫的血淋巴中表达。Mr-npc 2a的破坏减少了酵母样菌丝体细胞膜中的甾醇的量，从而促进在宿主体内的分散。因此，这些比野生型更容易受到昆虫免疫反应的影响。转基因表达Mr-NPC 2a增加了球孢白僵菌的毒力，球孢白僵菌是一种缺乏Mr-NPC 2a同源物的内生昆虫病原真菌。
Metarhizium robertsii is a plant root colonizing fungus that is also an insect pathogen. Its entomopathogenicity is a characteristic that was acquired during evolution from a plant endophyte ancestor. This transition provides a novel perspective on how new functional mechanisms important for host switching and virulence have evolved. From a random T-DNA insertion library, we obtained a pathogenicity defective mutant that resulted from the disruption of a sterol carrier gene (Mr-npc2a). Phylogenetic analysis revealed that Metarhizium acquired Mr-npc2a from an insect by horizontal gene transfer (HGT). Mr-NPC2a binds to cholesterol, an animal sterol, rather than the fungal sterol ergosterol, indicating it retains the specificity of insect NPC2 proteins. Mr-NPC2a is an intracellular protein and is exclusively expressed in the hemolymph of living insects. The disruption of Mr-npc2a reduced the amount of sterol in cell membranes of the yeast-like hyphal bodies that facilitate dispersal in the host body. These were consequently more susceptible to insect immune responses than the wild type. Transgenic expression of Mr-NPC2a increased the virulence of Beauveria bassiana, an endophytic insect-pathogenic fungus that lacks a Mr-NPC2a homolog.
登录
查看更多内容
影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
3
作者：
Fang, Weiguo;Pava-Ripoll, Monica;Leger, Raymond St.
通讯作者：
Leger, Raymond St.
影响因子：
56.9
作者：
Davis, CC;Wurdack, KJ
通讯作者：
Wurdack, KJ
影响因子：
30.8
作者：
Friesen, Timothy L.;Stukenbrock, Eva H.;Oliver, Richard P.
通讯作者：
Oliver, Richard P.
影响因子：
3.4
作者：
Fang, Weiguo;Feng, Jin;Pei, Yan
通讯作者：
Pei, Yan