Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens.
Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens.
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DOI：
10.1016/j.celrep.2023.112397
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发表时间：
2023-05-30
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
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兴奋性突触通常被描述为单突触终扣（SSB），其中一个突触前终扣接触单个突触后棘。使用连续切片块面扫描电子显微镜，我们发现，这本教科书定义的突触并不完全适用于海马CA1区。大约有一半的兴奋性突触在层oriens涉及多突触终扣（MSB），其中一个单一的突触前终扣包含多个活动区接触许多突触后棘（从2到7）的基底树突上的不同细胞。在发育过程中（从出生后第22天[P22]到P100），MSB的比例增加，并随着与索马的距离而减少。奇怪的是，突触特性，如活动区（AZ）或突触后密度（PSD）的大小表现出较少的MSB内的变化相比，相邻的SSB，超分辨率光学显微镜证实的功能。计算机模拟表明，这些属性有利于在CA 1网络的同步活动。CA1基底树突上的突触中有一半是多突触终扣（MSB）。每个MSB可以接触来自不同细胞的多达7个树突棘。由同一MSB形成的突触具有相似的形态学特性。显示在定向层中海马CA1基底树突上形成的所有突触中大约有一半是多突触终扣（MSB）：由一个突触前终扣形成在来自不同细胞的多个突触后棘上的兴奋性突触。它们的数量和形态学特性表明，它们可能有助于促进网络同步。
Excitatory synapses are typically described as single synaptic boutons (SSBs), where one presynaptic bouton contacts a single postsynaptic spine. Using serial section block-face scanning electron microscopy, we found that this textbook definition of the synapse does not fully apply to the CA1 region of the hippocampus. Roughly half of all excitatory synapses in the stratum oriens involved multi-synaptic boutons (MSBs), where a single presynaptic bouton containing multiple active zones contacted many postsynaptic spines (from 2 to 7) on the basal dendrites of different cells. The fraction of MSBs increased during development (from postnatal day 22 [P22] to P100) and decreased with distance from the soma. Curiously, synaptic properties such as active zone (AZ) or postsynaptic density (PSD) size exhibited less within-MSB variation when compared with neighboring SSBs, features that were confirmed by super-resolution light microscopy. Computer simulations suggest that these properties favor synchronous activity in CA1 networks. Half of all synapses onto CA1 basal dendrites are multi-synaptic boutons (MSBs) Each MSB can contact up to 7 dendritic spines from different cells Synapses made by the same MSB share similar morphological properties Simulations suggest MSBs promote network synchrony Ribgy et al. show that roughly half of all synapses formed onto hippocampal CA1 basal dendrites in stratum oriens are multi-synaptic boutons (MSBs): excitatory synapses formed by one presynaptic bouton onto multiple postsynaptic spines from distinct cells. Their large numbers and morphological properties suggest that they may help promote network synchrony.
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