The Synapse Diversity Dilemma: Molecular Heterogeneity Confounds Studies of Synapse Function.
The Synapse Diversity Dilemma: Molecular Heterogeneity Confounds Studies of Synapse Function.
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DOI：
10.3389/fnsyn.2020.590403
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发表时间：
2020
影响因子：
3.7
通讯作者：
Fransén E
中科院分区：
文献类型：
--
作者：
Grant SGN;Fransén E
最近的研究表明，由于个体突触之间的分子和形态差异而产生了出乎意料的高度突触多样性。不同的突触类型在空间上分布在单个树突内、不同神经元之间以及大脑区域之间，从而产生大脑的突触体结构。突触异质性的空间组织很重要，因为异质兴奋性突触的生理激活会产生突触电位的不均匀空间输出，这会混淆从群体平均电极、光极和缺乏单突触分辨率的生化方法获得的测量结果的解释。群体平均测量无法区分突触群体组成的变化和突触生理学的变化。在这里，我们考虑突触多样性及其组织成突触组结构的影响，以研究突触生理学、可塑性、发育和行为，以及解释药理学和遗传扰动产生的表型。我们的结论是，基于群体平均测量的流行模型需要重新考虑，并且需要单突触分辨率生理记录方法来确认或反驳主要的突触行为模型。
Recent studies have shown an unexpectedly high degree of synapse diversity arising from molecular and morphological differences among individual synapses. Diverse synapse types are spatially distributed within individual dendrites, between different neurons, and across and between brain regions, producing the synaptome architecture of the brain. The spatial organization of synapse heterogeneity is important because the physiological activation of heterogeneous excitatory synapses produces a non-uniform spatial output of synaptic potentials, which confounds the interpretation of measurements obtained from population-averaging electrodes, optrodes and biochemical methods that lack single-synapse resolution. Population-averaging measurements cannot distinguish between changes in the composition of populations of synapses and changing synaptic physiology. Here we consider the implications of synapse diversity and its organization into synaptome architecture for studies of synapse physiology, plasticity, development and behavior, and for the interpretation of phenotypes arising from pharmacological and genetic perturbations. We conclude that prevailing models based on population-averaging measurements need reconsideration and that single-synapse resolution physiological recording methods are required to confirm or refute the major synaptic models of behavior.
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影响因子：
4.6
作者：
Broadhead MJ;Horrocks MH;Zhu F;Muresan L;Benavides-Piccione R;DeFelipe J;Fricker D;Kopanitsa MV;Duncan RR;Klenerman D;Komiyama NH;Lee SF;Grant SG
通讯作者：
Grant SG
影响因子：
16.6
作者：
Bayés À;Collins MO;Reig-Viader R;Gou G;Goulding D;Izquierdo A;Choudhary JS;Emes RD;Grant SG
通讯作者：
Grant SG
影响因子：
4.7
作者：
Frank RAW;Zhu F;Komiyama NH;Grant SGN
通讯作者：
Grant SGN
影响因子：
64.5
作者：
Altschuler SJ;Wu LF
通讯作者：
Wu LF
影响因子：
16.2
作者：
Micheva, Kristina D.;Busse, Brad;Weiler, Nicholas C.;O'Rourke, Nancy;Smith, Stephen J.
通讯作者：
Smith, Stephen J.