Axospinous synaptic subtype-specific differences in structure, size, ionotropic receptor expression, and connectivity in apical dendritic regions of rat hippocampal CA1 pyramidal neurons.
Axospinous synaptic subtype-specific differences in structure, size, ionotropic receptor expression, and connectivity in apical dendritic regions of rat hippocampal CA1 pyramidal neurons.
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DOI：
10.1002/cne.21896
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发表时间：
2009-01-20
影响因子：
2.5
通讯作者：
Geinisnian, Yuri
中科院分区：
文献类型：
--
作者：
Nicholson, Daniel A.;Geinisnian, Yuri
关键词：
轴棘突触及其亲本棘细胞的形态差异很大。此外，这些突触中的许多被多个突触连接，并且在受体的表达上显示出很大的差异性。轴棘突触的两个主要亚型是穿孔和非穿孔，但在这两类中有几个亚类。本研究用连续切片电子显微镜观察穿孔突触亚型和非穿孔突触亚型在大鼠海马区CA1区三个顶端树突区：放射层的近1/3、远侧1/3和腔隙分子层中的分布、大小、受体表达和与MSB的连接性方面是否存在差异。所有突触亚型都存在于顶端树突区域，但有几个亚类特有的差异。首先，分段的、完全分割的突触的数量、比例和AMPA受体的表达随着距离胞体的距离而改变，超过了在其他穿孔突触亚型中发现的距离。第二，不典型的大型非穿孔突触表现出与穿孔突触相同的NMDA受体免疫反应性，AMPA受体的表达水平介于非穿孔突触和穿孔突触之间，结构上穿孔突触样变化随距离胞体的距离而变化。最后，近侧放射层的MSB连通性最高，但仅限于由非穿孔突触组成的MSB。然而，免疫金数据表明，大多数MSB不会在多个神经元或脊髓中同时产生去极化，因为绝大多数MSB由两个受体表达异常低的突触组成，或者涉及一个受体表达高水平的突触和另一个受体表达水平低的突触。
The morphology of axospinous synapses and their parent spines varies widely. Additionally, many of these synapses are contacted by multiple synapse boutons (MSBs) and show substantial variability in receptor expression. The two major axospinous synaptic subtypes are perforated and nonperforated, but there are several subcategories within these two classes. The present study used serial section electron microscopy to determine whether perforated and nonperforated synaptic subtypes differed with regard to their distribution, size, receptor expression, and connectivity to MSBs in three apical dendritic regions of rat hippocampal area CA1: the proximal and distal thirds of stratum radiatum, and stratum lacunosum-moleculare. All synaptic subtypes were present throughout the apical dendritic regions, but there were several subclass-specific differences. First, segmented, completely partitioned synapses changed in number, proportion, and AMPA receptor expression with distance from the soma beyond that found within other perforated synaptic subtypes. Second, atypically large nonperforated synapses showed NMDA receptor immunoreactivity identical to perforated synapses, levels of AMPA receptor expression intermediate to nonperforated and perforated synapses, and perforated synapse-like changes in structure with distance from the soma. Finally, MSB connectivity was highest in proximal stratum radiatum, but only for those MSBs comprised of nonperforated synapses. The immunogold data suggest that most MSBs would not generate simultaneous depolarizations in multiple neurons or spines, however, because the vast majority of MSBs are comprised of two synapses with abnormally low levels of receptor expression, or involve one synapse with a high level of receptor expression and another with only a low level.
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影响因子：
1.7
作者：
Desmond, NL;Weinberg, RJ
通讯作者：
Weinberg, RJ
DOI：
10.1083/jcb.78.1.36
发表时间：
1978-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cohen RS;Siekevitz P
通讯作者：
Siekevitz P
DOI：
10.1073/pnas.0630290100
发表时间：
2003-04-15
影响因子：
11.1
作者：
Conti, R;Lisman, J
通讯作者：
Lisman, J
影响因子：
2.9
作者：
GEINISMAN, Y;MORRELL, F;DETOLEDOMORRELL, L
通讯作者：
DETOLEDOMORRELL, L
DOI：
10.1007/bf02284843
发表时间：
1996-12-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
Geinisman, Y;Gundersen, HJG;West, MJ
通讯作者：
West, MJ