The CD3 versus CD7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with HTLV-I.
The CD3 versus CD7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with HTLV-I.
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DOI：
10.1371/journal.pone.0053728
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Uchimaru K
中科院分区：
文献类型：
--
作者：
Kobayashi S;Tian Y;Ohno N;Yuji K;Ishigaki T;Isobe M;Tsuda M;Oyaizu N;Watanabe E;Watanabe N;Tani K;Tojo A;Uchimaru K
在最近的一项研究中，通过流式细胞术从急性型患者中纯化成人T细胞白血病淋巴瘤（ATL）细胞，在CD 3与CD 7图中观察到三个亚群（H：CD 3 highCD 7 high; D：CD 3dimCD 7 dim; L：CD 3dimCD 7 low）。大多数白血病细胞富集在L亚群中，并且相同的克隆包括在D和L亚群中，表明克隆进化。在这项研究中，我们分析了惰性型ATL和人类T细胞白血病病毒I型（HTLV-I）无症状携带者（AC）的患者，以了解CD 3与CD 7的特征是否反映了HTLV-I感染细胞特性的进展。使用来自患者样本的外周血单核细胞，我们进行多色流式细胞术。对经历荧光激活细胞分选的细胞进行分子分析，包括反向长PCR。在D（%）与L（%）图中，患者数据主要可分为三组（第1组：AC;第2组：阴燃型和慢性型ATL;第3组：急性型ATL）。但也注意到一些例外情况（例如，第2组中的AC）。在一些患者的随访中，临床疾病进展与CD 3与CD 7特征密切相关。在克隆性分析中，我们在ATL病例的D和L亚群中清楚地检测到一个主要克隆，有趣的是，在第2组的一些AC中。我们认为，CD 3与CD 7曲线反映了HTLV-I感染患者疾病阶段的进展。CD 3与CD 7谱将与高前病毒载量沿着成为HTLV-I AC预测疾病进展的新指标。
In a recent study to purify adult T-cell leukemia-lymphoma (ATL) cells from acute-type patients by flow cytometry, three subpopulations were observed in a CD3 versus CD7 plot (H: CD3highCD7high; D: CD3dimCD7dim; L: CD3dimCD7low). The majority of leukemia cells were enriched in the L subpopulation and the same clone was included in the D and L subpopulations, suggesting clonal evolution. In this study, we analyzed patients with indolent-type ATL and human T-cell leukemia virus type I (HTLV-I) asymptomatic carriers (ACs) to see whether the CD3 versus CD7 profile reflected progression in the properties of HTLV-I-infected cells. Using peripheral blood mononuclear cells from patient samples, we performed multi-color flow cytometry. Cells that underwent fluorescence-activated cell sorting were subjected to molecular analyses, including inverse long PCR. In the D(%) versus L(%) plot, patient data could largely be categorized into three groups (Group 1: AC; Group 2: smoldering- and chronic-type ATL; and Group 3: acute-type ATL). Some exceptions, however, were noted (e.g., ACs in Group 2). In the follow-up of some patients, clinical disease progression correlated well with the CD3 versus CD7 profile. In clonality analysis, we clearly detected a major clone in the D and L subpopulations in ATL cases and, intriguingly, in some ACs in Group 2. We propose that the CD3 versus CD7 plot reflects progression of disease stage in patients infected with HTLV-I. The CD3 versus CD7 profile will be a new indicator, along with high proviral load, for HTLV-I ACs in forecasting disease progression.
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影响因子：
4.8
作者：
Sasaki, Daisuke;Doi, Yuko;Kamihira, Shimeru
通讯作者：
Kamihira, Shimeru
DOI：
10.1073/pnas.87.17.6713
发表时间：
1990-09-01
影响因子：
11.1
作者：
WILLARDGALLO, KE;VANDEKEERE, F;KETTMANN, R
通讯作者：
KETTMANN, R
无症状 HTLV-1 携带者的人类 T 细胞白血病病毒 I 型（HTLV-1）前病毒载量和疾病进展：日本的一项全国性前瞻性研究
影响因子：
20.3
作者：
Iwanaga, Masako;Watanabe, Toshiki;Yamaguchi, Kazunari
通讯作者：
Yamaguchi, Kazunari
影响因子：
45.3
作者：
Tsukasaki, Kunihiro;Hermine, Olivier;Watanabe, Toshiki
通讯作者：
Watanabe, Toshiki
影响因子：
6.5
作者：
YAMAGUCHI, K;KIYOKAWA, T;TAKATSUKI, K
通讯作者：
TAKATSUKI, K