Multiple levels of regulation of protein synthesis at fertilization in sea urchin eggs.
Multiple levels of regulation of protein synthesis at fertilization in sea urchin eggs.
复制标题
海胆卵受精时蛋白质合成的多水平调控。
DOI：
10.1016/0012-1606(85)90312-4
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发表时间：
1985
影响因子：
2.7
通讯作者：
Hershey,JW
中科院分区：
文献类型：
--
作者：
Winkler,MM;Nelson,EM;Lashbrook,C;Hershey,JW
海胆卵的受精导致蛋白质合成的大刺激。蛋白质合成的增加是由储存的母体mRNA（mRNP）动员到多聚核糖体中介导的，但调节这一过程的分子机制的细节尚不清楚。使用海胆卵无细胞翻译系统，已经获得的证据表明，启动新mRNA的蛋白质合成的能力是有限的。添加外源性mRNA未能刺激整体蛋白质合成，而补充系统与核酸酶处理的网织红细胞裂解物，S-100上清液馏分，或纯化的eIF-2刺激近两倍。此外，含有40 S核糖体亚基和甲硫氨酰-tRNA的43 S preinitiation复合物的水平在pH 7.4时比pH 6.9时或加入网织红细胞S-100时增加。然而，其他实验清楚地表明，mRNA的可用性也可能调节海胆卵中的翻译。海胆裂解物仅刺激核酸酶处理的网织红细胞裂解物系统较差，并且海胆裂解物中的mRNP不与网织红细胞43 S前起始复合物结合。由于纯化的海胆卵mRNA在两种测定中都有活性，因此海胆卵中的大部分mRNA必须被掩蔽，并且在体外测定中保持掩蔽。因此，蛋白质合成似乎在mRNA可用性和翻译机器组件的活性水平上受到调节。
Fertilization of sea urchin eggs results in a large stimulation of protein synthesis. This increase in protein synthesis is mediated by the mobilization of stored maternal mRNA (mRNPs) into polysomes, but the details of the molecular mechanisms which regulate this process are not well understood. Using a sea urchin egg cell-free translation system, evidence has been obtained which indicates that the capacity to initiate protein synthesis on new mRNAs is limited. Addition of exogenous mRNAs failed to stimulate overall protein synthesis, whereas supplementing the system with a nuclease-treated reticulocyte lysate, an S-100 supernatant fraction, or purified eIF-2 stimulated nearly twofold. In addition, the levels of 43 S preinitiation complexes containing a 40 S ribosomal subunit and methionyl-tRNA were increased at pH 7.4 compared to pH 6.9, or when reticulocyte S-100 was added. However, other experiments showed clearly that mRNA availability may also regulate translation in the sea urchin egg. Sea urchin lysates only stimulated poorly the nuclease-treated reticulocyte lysate system, and the mRNPs in the sea urchin lysate did not bind to reticulocyte 43 S preinitiation complexes. Since purified sea urchin egg mRNA was active in both assays, the bulk of sea urchin mRNA must be masked in the egg, and remain masked in thein vitroassays. Thus, protein synthesis appears to be regulated at both the level of mRNA availability and the activity of components of the translational machinery.
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影响因子：
64.8
作者：
J. D. Johnson;D. Epel;M. Paul
通讯作者：
M. Paul
活爪蟾卵母细胞中信使 RNA 的竞争。
DOI：
10.1111/j.1432-1033.1979.tb12892.x
发表时间：
1979
期刊：
European journal of biochemistry
影响因子：
--
作者：
F. Asselbergs;W. V. van Venrooij;H. Bloemendal
通讯作者：
H. Bloemendal
对屏蔽信息假说的评估：海胆卵信使核糖核蛋白复合物是体外蛋白质合成的有效模板。
影响因子：
2.7
作者：
R. Moon;M. Danilchik;M. B. Hille
通讯作者：
M. B. Hille
影响因子：
64.8
作者：
M. Winkler;R. Steinhardt;J. L. Grainger;L. Minning
通讯作者：
M. Winkler;R. Steinhardt;J. L. Grainger;L. Minning
影响因子：
64.5
作者：
LASKEY, RA;MILLS, AD;PARTINGTON, GA
通讯作者：
PARTINGTON, GA