Highly transcribed RNA polymerase II genes are impediments to replication fork progression in Saccharomyces cerevisiae.
Highly transcribed RNA polymerase II genes are impediments to replication fork progression in Saccharomyces cerevisiae.
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DOI：
10.1016/j.molcel.2009.05.022
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发表时间：
2009-06-26
期刊：
影响因子：
16
通讯作者：
Zakian, Virginia A.
中科院分区：
文献类型：
--
作者：
Azvolinsky, Anna;Giresi, Paul G.;Lieb, Jason D.;Zakian, Virginia A.
复制分叉面临着多种阻碍其进展的障碍。通过二维凝胶分析，酵母叉暂停在稳定的DNA蛋白质复合物，这种暂停大大增加在Rrm3解旋酶的情况下。我们使用全基因组方法鉴定了野生型细胞中96个非常高的DNA聚合酶结合位点。这些结合位点中的大多数不是先前鉴定的暂停位点。相反，在高DNA聚合酶结合位点中，最具代表性的基因组类别是高度转录的RNA聚合酶II基因的开放阅读框（ORF）。与野生型细胞相比，在rrm3中鉴定了两倍的暂停位点，因为在该菌株中的暂停发生在高度转录的RNA聚合酶II基因和先前鉴定的蛋白质DNA复合物处。高度转录的RNA聚合酶II基因的ORF是第一类在rrm3细胞中不加剧的自然暂停位点。
Replication forks face multiple obstacles that slow their progression. By two-dimensional gel analysis, yeast forks pause at stable DNA protein complexes, and this pausing is greatly increased in the absence of the Rrm3 helicase. We used a genome wide approach to identify 96 sites of very high DNA polymerase binding in wild type cells. Most of these binding sites were not previously identified pause sites. Rather, the most highly represented genomic category among high DNA polymerase binding sites was the open reading frames (ORFs) of highly transcribed RNA polymerase II genes. Twice as many pause sites were identified in rrm3 compared to wild type cells as pausing in this strain occurred at both highly transcribed RNA polymerase II genes and the previously identified protein DNA complexes. ORFs of highly transcribed RNA polymerase II genes are the first class of natural pause sites that are not exacerbated in rrm3 cells.
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