A synthetic cartilage extracellular matrix model: hyaluronan and collagen hydrogel relaxivity, impact of macromolecular concentration on dGEMRIC.
A synthetic cartilage extracellular matrix model: hyaluronan and collagen hydrogel relaxivity, impact of macromolecular concentration on dGEMRIC.
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DOI：
10.1007/s00256-011-1331-z
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发表时间：
2012-02
影响因子：
2.1
通讯作者：
Calabro, Anthony
中科院分区：
文献类型：
--
作者：
Laurens, Ediuska;Schneider, Erika;Winalski, Carl S.;Calabro, Anthony
开发和表征使用水凝胶的软骨细胞外基质合成模型的MR特性，并确定在存在和不存在用于dGEMRIC的钆喷酸葡胺（Gd-DTPA）的情况下，水凝胶及其糖胺聚糖和胶原组分的自旋-晶格（T1）和自旋-自旋（T2）弛豫时间的浓度依赖性。T1和T2测量是在3特斯拉下对一系列明胶（即，胶原）和透明质酸（即，葡糖胺聚糖）溶液（6.25-100 g/l），单独，在复合物中一起，以及作为二酪胺桥接的水凝胶。弛豫率作为大分子浓度的函数计算。即使在最高浓度下，明胶和透明质酸溶液的T1和T2值也显著大于软骨报告的值。只有在软骨中天然存在的明胶和透明质酸浓度的复合水凝胶产生T1值，但不产生代表软骨的T2值。松弛度略微依赖于单独的透明质酸浓度（R1= 0.0027 l g−1 s−1; R2=0.025 l g−1 s−1）和明胶浓度（R1=0.0032 l g−1 s−1; R2=0.020 l g−1 s−1）以及作为复合物（R1=0.0068 l g−1 s−1; R2=0.101 l g−1 s−1）。Gd-DTPA弛豫率取决于大分子浓度，在软骨生物化学范围内变化14-32%（R1=4.24 - 5.55 mM-1 s-1; R2=4.60 - 6.27 mM-1 s-1）。在没有造影剂的情况下，单独或复合的透明质酸和明胶对模型系统的弛豫率具有非常小的影响。对R1的影响比对R2的影响小大约10倍。相比之下，高于50 g/l的大分子浓度显著影响Gd-DTPA弛豫率，当使用dGEMRIC测量体内软骨的糖胺聚糖含量时，应考虑到这一点。
To develop and characterize the MR properties of a synthetic model for cartilage extracellular matrix using hydrogels and to determine the concentration dependence of spin–lattice (T1) and spin-spin (T2) relaxation times of hydrogels and their glycosaminoglycan and collagen components in the presence and absence of gadopentetate dimeglumine (Gd-DTPA) for use in dGEMRIC. T1 and T2 measurements were made at 3 Tesla on a range of gelatin (i.e., collagen) and hyaluronan (i.e., glycosaminoglycan) solutions (6.25–100 g/l), alone, together in a composite, and as dityramine-bridged hydrogels. Relaxivity was calculated as a function of macro-molecular concentration. Even at the highest concentrations, gelatin and hyaluronan solutions had T1 and T2 values significantly larger than those reported for cartilage. Only composite hydrogels with gelatin and hyaluronan concentrations naturally found in cartilage resulted in T1 values, but not T2 values, representative of cartilage. Relaxivities were slightly dependent on both hyaluronan concentration (R1= 0.0027 l g−1 s−1; R2=0.025 l g−1 s−1) and gelatin concentration (R1=0.0032 l g−1 s−1; R2=0.020 l g−1 s−1) alone and as a composite (R1=0.0068 l g−1 s−1; R2=0.101 l g−1 s−1). Gd-DTPA relaxivities were dependent upon macromolecular concentration and varied by 14–32% (R1=4.24 to 5.55 mM−1 s−1; R2=4.60 to 6.27 mM−1 s−1) over the range of cartilage biochemistry. Without the contrast agent, hyaluronan and gelatin, alone or in a composite, have a very small impact on the relaxivities of the model system. The impact on R1 was approximately tenfold less than on R2. In contrast, macromolecular concentrations above 50 g/l significantly impacted Gd-DTPA relaxivity and should be accounted for when measuring the glycosaminoglycan content of cartilage in vivo using dGEMRIC.
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影响因子：
27.4
作者：
Eckstein, F.;Le Graverand, M. P. Hellio;Buck, R.
通讯作者：
Buck, R.
影响因子：
4.4
作者：
Li, Wei;Edelman, Robert R.;Prasad, Pottumarthi V.
通讯作者：
Prasad, Pottumarthi V.
影响因子：
3.3
作者：
Gillis, A;Gray, M;Burstein, D
通讯作者：
Burstein, D
影响因子：
3.3
作者：
Bittersohl, Bernd;Hosalkar, Harish S.;Mamisch, Tallal C.
通讯作者：
Mamisch, Tallal C.
影响因子：
5
作者：
Gold, GE;Han, E;Beaulieu, C
通讯作者：
Beaulieu, C