Expression characteristics of two ubiquitin/ribosomal fusion protein genes in the developing testis, accessory gonad and ovary of Chinese mitten crab, Eriocheir sinensis
Expression characteristics of two ubiquitin/ribosomal fusion protein genes in the developing testis, accessory gonad and ovary of Chinese mitten crab, Eriocheir sinensis
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两种泛素/核糖体融合蛋白基因在中华绒螯蟹发育中睾丸、副性腺和卵巢中的表达特征
DOI：
10.1007/s11033-012-1474-6
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发表时间：
2012-02
影响因子：
2.8
通讯作者：
Jiang, Hui
中科院分区：
文献类型：
--
作者：
Wang, Qun;Chen, Lili;Wang, Ying;Li, Weiwei;He, Lin;Jiang, Hui
泛素/蛋白酶体途径在配子发生中起着重要作用。泛素/核糖体融合蛋白是单泛素的前体。我们在中华绒螯蟹精巢中发现了两个泛素/核糖体融合蛋白基因，泛素-核糖体融合蛋白S27和L40分别命名为UBS27和UBL40。根据从睾丸cDNA文库中分离到的初始表达序列标签(EST)和3‘RACE结果，用聚合酶链式反应(PCR)克隆了Es-UBS27cDNA全长(565hp)。该序列包含一个465个核苷酸的开放阅读框(ORF)，编码154个氨基酸的蛋白质。它含有泛素(UB)结构域和核糖体蛋白S27。全长Es-UBL40cDNA(521hp)包含390个核苷酸的ORF，编码129个氨基酸的蛋白质。它含有一个UB结构域和核糖体蛋白L40。实时荧光定量聚合酶链式反应显示，这两个UB基因在发育中的精巢和卵巢中的表达水平发生了显著变化，分别在8月和10月达到表达高峰。在附性腺季节性发育过程中，UBS27和UBL40的表达变化剧烈，在11月份达到表达高峰。睾丸和卵巢的原位杂交表明，UB转录广泛发生。我们的研究首次证明了UBS27和UBL40在中华绒螯蟹配子发生和繁殖成功中起关键作用。
The ubiquitin/proteasome pathway plays an important role in gametogenesis. Ubiquitin/ribosomal fusion protein is the precursor of mono-ubiquitin. We identified two ubiquitin/ribosomal fusion proteins genes in testis ofEriocheir sinensis.The ubiquitin-ribosomal proteins S27 and L40 are denoted as UBS27 and UBL40, respectively. The full lengthEs-UBS27cDNA (565 bp) was cloned using PCR, based upon an initial expressed sequence tag (EST) isolated from a testicular cDNA library and the result of 3′ RACE. The sequence contained a 465-nucleotide open reading frame (ORF) that encoded a protein of 154 amino acids. It contained a Ubiquitin (UB) domain and the ribosomal protein S27. The full-lengthEs-UBL40cDNA (521 bp) contained a 390-nucleotide ORF that encoded a protein of 129 amino acids. It contained a UB domain and the ribosomal protein L40. By real-time PCR, the expression level of these two UB genes is shown to change significantly in the developing testis and ovary, reaching peak expression in August and October, respectively. During accessory gonad seasonal development, the expression ofUBS27andUBL40varied sharply and reached peak expression in November. In situ hybridization of testis and ovary, indicated that UB transcription took place widely. Our study demonstrates, for the first time, that UBS27 and UBL40 play key roles in gametogenesis and reproductive success inEriocheir sinensis.
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手指蟹睾丸（Eriocheir sinensis）的转录组分析。
影响因子：
2.1
作者：
Zhang W;Wan H;Jiang H;Zhao Y;Zhang X;Hu S;Wang Q
通讯作者：
Wang Q
影响因子：
10.5
作者：
Cenci, G;Rawson, RB;Gatti, M
通讯作者：
Gatti, M
DOI：
10.1016/s0021-9258(18)34958-5
发表时间：
1982-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Haas;J. V. Warms;A. Hershko;I. A. Rose
通讯作者：
A. Haas;J. V. Warms;A. Hershko;I. A. Rose
影响因子：
2.2
作者：
A. Moura;D. Chapman;H. Koc;G. Killian
通讯作者：
A. Moura;D. Chapman;H. Koc;G. Killian
影响因子：
3.5
作者：
J. Mezquita;B. López-Ibor;M. Pau;C. Mezquita
通讯作者：
J. Mezquita;B. López-Ibor;M. Pau;C. Mezquita