Matrix metalloproteinases (MMPs) in the endometrium of bitches.
Matrix metalloproteinases (MMPs) in the endometrium of bitches.
复制标题
母犬子宫内膜中的基质金属蛋白酶（MMP）。
作者：
Po;L. Salamonsen;C. S. Lee;P. Wright
本实验研究了犬子宫基质金属蛋白酶（MMPs）活性与子宫腔上皮变性、子宫内膜囊性增生、子宫积脓及产后子宫重塑的关系。对成熟母犬（n = 10）进行卵巢切除，用激素（苯甲酸雌二醇、孕激素）处理，并在模拟发情前期（n = 2）、发情期（n = 2）、发情间期（n = 2）、发情中期（n = 2）和发情后期（n = 2）（分别在孕激素停止处理后3周和9周）进行研究。未给药母犬（n = 1/组）作为对照（实验1）。在处理诱导的模拟间情期结束时，另外10只卵巢切除母犬每天接受相同剂量（标准剂量，n = 3）、降低剂量（n = 3）或增加剂量（n = 3）的孕激素处理，或不进行处理（停药剂量，n = 1），持续3周。然后对这些母犬进行研究（实验2）。在卵巢切除术时，将缝线放置在另外5只母犬的一个子宫角腔内。对其中3只母犬进行处理以诱导模拟间情期，2只母犬作为未处理对照。在经阴道给药的母犬中，缝合导致1只母犬出现囊性子宫内膜增生，2只母犬出现囊性子宫内膜增生伴子宫积脓。对照组母犬未显示囊性子宫内膜增生或子宫积脓（实验3）。在产后2周（n = 1）、3周（n = 2）和11周（n = 1）对4只完整母犬进行了研究。还从两只患有自然发生的囊性子宫内膜增生伴子宫积脓的母犬中采集子宫组织（实验4）。对所有子宫进行组织学检查，并使用子宫内膜提取物的酶谱法评估MMP-2、MMP-7和MMP-9（潜伏型和活性型）的活性。在试验1和试验2中，6/25只母犬（模拟发情中期、停药剂量组和剂量降低组）的管腔上皮显著变性与MMP活性变化无关。MMP-2（活性形式）、MMP-7（潜伏形式）和MMP-9（活性和潜伏形式）的活性在患有子宫积脓的囊性子宫内膜增生的母犬（但不是单独的囊性子宫内膜增生）和产后2周和3周的母犬（但不是产后11周）中显著增加。这些结果表明，MMPs不参与腔上皮的变性，但参与产后母犬的子宫重塑和子宫内膜囊性增生伴子宫积脓。
The relationships between activities of matrix metalloproteinases (MMPs) in the canine uterus and the occurrence of degeneration of the luminal epithelium, cystic endometrial hyperplasia, pyometra and uterine remodelling post partum were determined. Mature bitches (n = 10) were ovariectomized, treated with hormones (oestradiol benzoate, progestagen) and investigated at stages simulating pro-oestrus (n = 2), oestrus (n = 2), dioestrus (n = 2), and mid- (n = 2) and late (n = 2) anoestrus (3 and 9 weeks, respectively, after cessation of treatment with progestagen). Untreated bitches (n = 1 per group) served as controls (Expt 1). An additional 10 ovariectomized bitches, at the end of treatment-induced simulated dioestrus, were treated each day for a further 3 weeks either with the same dose (standard dose, n = 3), a decreased dose (n = 3) or an increased dose (n = 3) of progestagen, or no treatment (withdrawal dose, n = 1). These bitches were then investigated (Expt 2). A suture was placed in the lumen of one uterine horn of another five bitches at ovariectomy. Three of these bitches were treated to induce simulated dioestrus and two bitches served as untreated controls. In the hormone-treated bitches, the suture resulted in cystic endometrial hyperplasia in one bitch and in cystic endometrial hyperplasia with pyometra in two bitches. The control bitches showed no cystic endometrial hyperplasia or pyometra (Expt 3). Four intact bitches were studied at 2 (n = 1), 3 (n = 2) and 11 (n = 1) weeks post partum. Uterine tissues were also collected from two bitches with naturally occurring cystic endometrial hyperplasia with pyometra (Expt 4). All uteri were examined histologically and the activities of MMP-2, -7 and -9 (latent and active forms) were assessed using zymography of extracts of endometrium. In Expts 1 and 2, marked degeneration of the luminal epithelium in six of 25 bitches (simulated mid-anoestrus, withdrawal dose and decreased dose groups) was not associated with changes in MMP activities. Markedly increased activities of MMP-2 (active form), -7 (latent form) and -9 (active and latent forms) were observed in the bitches with cystic endometrial hyperplasia with pyometra (but not with cystic endometrial hyperplasia alone) and in the bitches at 2 and 3 weeks post partum (but not at 11 weeks post partum). These results indicate that MMPs are not involved with degeneration of the luminal epithelium, but are involved with uterine remodelling in the postpartum bitch and with cystic endometrial hyperplasia with pyometra.
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大鼠子宫中基质溶解素的调节。
DOI：
10.1139/o96-084
发表时间：
1996
期刊：
Biochemistry and cell biology = Biochimie et biologie cellulaire
影响因子：
--
作者：
WoessnerJr,JF
通讯作者：
WoessnerJr,JF
人子宫内膜基质金属蛋白酶-2（一种假定的月经蛋白酶）--月经周期中激素对培养基质细胞的调控和信使 RNA 的表达
影响因子：
15.9
作者：
Irwin, JC;Kirk, D;Giudice, LC
通讯作者：
Giudice, LC
影响因子：
3.4
作者：
L. Salamonsen;D. Woolley
通讯作者：
L. Salamonsen;D. Woolley
影响因子：
15.9
作者：
RODGERS, WH;MATRISIAN, LM;OSTEEN, KG
通讯作者：
OSTEEN, KG
影响因子：
13.3
作者：
Salamonsen, LA;Lathbury, LJ
通讯作者：
Lathbury, LJ