A comparison of non-magnetic and magnetic beads for measuring IgG antibodies against Plasmodium vivax antigens in a multiplexed bead-based assay using Luminex technology (Bio-Plex 200 or MAGPIX).
A comparison of non-magnetic and magnetic beads for measuring IgG antibodies against Plasmodium vivax antigens in a multiplexed bead-based assay using Luminex technology (Bio-Plex 200 or MAGPIX).
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DOI：
10.1371/journal.pone.0238010
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Longley RJ
中科院分区：
文献类型：
--
作者：
Mazhari R;Brewster J;Fong R;Bourke C;Liu ZSJ;Takashima E;Tsuboi T;Tham WH;Harbers M;Chitnis C;Healer J;Ome-Kaius M;Sattabongkot J;Kazura J;Robinson LJ;King C;Mueller I;Longley RJ
使用Luminex® xMAP®技术的基于微珠的多重检测已成为许多领域的流行检测方法，用于检测针对感兴趣蛋白质的抗体，包括疟疾和最近的SARS-CoV-2/COVID-19。目前广泛使用的有两种形式：非磁珠或磁珠。缺乏关于使用这两种类型的微珠获得的结果的可比性以及在不同仪器上运行的测定的数据。虽然非磁性微珠只能在基于流动的仪器（如Luminex® 100/200™或Bio-Mix ® 200）上运行，但磁性微珠可以在这些仪器和较新的MAGPIX®仪器上运行。在这项研究中，我们利用一组纯化的重组间日疟原虫蛋白和疟疾流行区的样品，使用不同的珠和仪器组合来测量间日疟原虫特异性IgG应答。我们直接比较：i）非磁性珠粒对在Bio-Bio ® 200上运行的磁性珠粒，ii）磁性珠粒对在Bio-Bio ® 200上运行的磁性珠粒对在MAGPIX ®上运行的磁性珠粒，以及iii）非磁性珠粒对在Bio-Bio ® 200上运行的磁性珠粒。我们还对我们优化的测定进行了外部比较。我们观察到，针对我们的间日疟原虫蛋白质组测量的IgG抗体应答在我们的所有三个比较中是中等-强相关的（对于18/19种蛋白质，Pearson r>0.5），然而需要更高量的蛋白质来偶联至磁珠。我们的外部比较表明，使用相同的偶联珠在不同实验室中产生的结果也具有高度可比性（pearson r>0.7），特别是如果使用参考标准曲线。
Multiplexed bead-based assays that use Luminex® xMAP® technology have become popular for measuring antibodies against proteins of interest in many fields, including malaria and more recently SARS-CoV-2/COVID-19. There are currently two formats that are widely used: non-magnetic beads or magnetic beads. Data are lacking regarding the comparability of results obtained using these two types of beads, and for assays run on different instruments. Whilst non-magnetic beads can only be run on flow-based instruments (such as the Luminex® 100/200™ or Bio-Plex® 200), magnetic beads can be run on both these and the newer MAGPIX® instruments. In this study we utilized a panel of purified recombinant Plasmodium vivax proteins and samples from malaria-endemic areas to measure P. vivax-specific IgG responses using different combinations of beads and instruments. We directly compared: i) non-magnetic versus magnetic beads run on a Bio-Plex® 200, ii) magnetic beads run on the Bio-Plex® 200 versus MAGPIX® and iii) non-magnetic beads run on a Bio-Plex® 200 versus magnetic beads run on the MAGPIX®. We also performed an external comparison of our optimized assay. We observed that IgG antibody responses, measured against our panel of P. vivax proteins, were moderately-strongly correlated in all three of our comparisons (pearson r>0.5 for 18/19 proteins), however higher amounts of protein were required for coupling to magnetic beads. Our external comparison indicated that results generated in different laboratories using the same coupled beads are also highly comparable (pearson r>0.7), particularly if a reference standard curve is used.
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影响因子：
9.4
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影响因子：
2.2
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影响因子：
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影响因子：
2.2
作者：
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通讯作者：
MacLennan, Calman A.
利用来自不同地方性环境的多种疟原虫抗原和血清，优化了基于磁珠的测定法（Magpix（®）-luminex），以对疟疾的暴露进行免疫监测。
影响因子：
3
作者：
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通讯作者：
Perraut R