Bifidobacterium animalis subsp. lactis BB-12 Protects against Antibiotic-Induced Functional and Compositional Changes in Human Fecal Microbiome.
Bifidobacterium animalis subsp. lactis BB-12 Protects against Antibiotic-Induced Functional and Compositional Changes in Human Fecal Microbiome.
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DOI：
10.3390/nu13082814
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发表时间：
2021-08-17
期刊：
影响因子：
5.9
通讯作者：
Kane MA
中科院分区：
文献类型：
--
作者：
Merenstein D;Fraser CM;Roberts RF;Liu T;Grant-Beurmann S;Tan TP;Smith KH;Cronin T;Martin OA;Sanders ME;Lucan SC;Kane MA
广谱抗生素的施用通常与腹泻相关性腹泻（AAD）相关，并且影响胃肠道稳态，如以下所证明的：（a）肠道微生物群的数量和多样性的总体减少，以及（B）短链脂肪酸（SCFA）产生减少。在人类中，益生菌可以增强抗生素治疗后微生物群种群的恢复的证据是模棱两可的，很少有研究讨论益生菌是否可以改善微生物代谢功能的恢复。我们的目的是确定是否动物双歧杆菌亚种。含有乳酸菌BB-12（BB-12）的酸奶可以防止益生菌诱导的粪便SCFA和微生物群组成破坏。我们进行了一项随机、分配隐藏、对照试验，阿莫西林/克拉维酸给药（第1-7天），同时给予含BB-12或对照酸奶（第1-14天）。我们在基线和第7、14、21和30天测量了SCFA和细菌组成的粪便水平。42名参与者被随机分配到BB-12组，20名参与者被分配到对照组。抗生素治疗抑制了对照组和益生菌组的粪便乙酸盐水平。在抗生素停止后，益生菌组的粪便乙酸盐水平在研究的剩余时间内增加，并在第30天恢复到基线水平（-1.6%基线），而在对照组中，乙酸盐水平仍然受到抑制。此外，抗生素治疗降低了所有研究参与者在第7天肠道微生物群的香农多样性。与益生菌组相比，对照组的这种变化幅度更大且更持续，这与BB-12增强微生物群恢复的假设一致。两组之间没有显著的基线临床差异。与对照组相比，对健康受试者同时给予阿莫西林/克拉维汀和BB-12酸奶与粪便SCFA水平的显著较小降低以及随时间推移更稳定的微生物群分类特征相关。
The administration of broad-spectrum antibiotics is often associated with antibiotic-associated diarrhea (AAD), and impacts gastrointestinal tract homeostasis, as evidenced by the following: (a) an overall reduction in both the numbers and diversity of the gut microbiota, and (b) decreased short-chain fatty acid (SCFA) production. Evidence in humans that probiotics may enhance the recovery of microbiota populations after antibiotic treatment is equivocal, and few studies have addressed if probiotics improve the recovery of microbial metabolic function. Our aim was to determine if Bifidobacterium animalis subsp. lactis BB-12 (BB-12)-containing yogurt could protect against antibiotic-induced fecal SCFA and microbiota composition disruptions. We conducted a randomized, allocation-concealed, controlled trial of amoxicillin/clavulanate administration (days 1–7), in conjunction with either BB-12-containing or control yogurt (days 1–14). We measured the fecal levels of SCFAs and bacterial composition at baseline and days 7, 14, 21, and 30. Forty-two participants were randomly assigned to the BB-12 group, and 20 participants to the control group. Antibiotic treatment suppressed the fecal acetate levels in both the control and probiotic groups. Following the cessation of antibiotics, the fecal acetate levels in the probiotic group increased over the remainder of the study and returned to the baseline levels on day 30 (−1.6% baseline), whereas, in the control group, the acetate levels remained suppressed. Further, antibiotic treatment reduced the Shannon diversity of the gut microbiota, for all the study participants at day 7. The magnitude of this change was larger and more sustained in the control group compared to the probiotic group, which is consistent with the hypothesis that BB-12 enhanced microbiota recovery. There were no significant baseline clinical differences between the two groups. Concurrent administration of amoxicillin/clavulanate and BB-12 yogurt, to healthy subjects, was associated with a significantly smaller decrease in the fecal SCFA levels and a more stable taxonomic profile of the microbiota over time than the control group.
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影响因子：
4.8
作者：
Kirkpatrick SI;Reedy J;Krebs-Smith SM;Pannucci TE;Subar AF;Wilson MM;Lerman JL;Tooze JA
通讯作者：
Tooze JA
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
7.6
作者：
Beaugerie, L;Flahault, A;Petit, JC
通讯作者：
Petit, JC
影响因子：
29.4
作者：
CLAUSEN, MR;BONNEN, H;MORTENSEN, PB
通讯作者：
MORTENSEN, PB
影响因子：
1.9
作者：
HOVERSTAD, T;CARLSTEDTDUKE, B;STEINBAKK, M
通讯作者：
STEINBAKK, M