Studies of the regulation of mouse renin genes by measurement of renin messenger ribonucleic acid.
Studies of the regulation of mouse renin genes by measurement of renin messenger ribonucleic acid.
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通过测量肾素信使核糖核酸研究小鼠肾素基因的调节。
DOI：
10.1210/endo-117-3-872
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发表时间：
1985
期刊：
影响因子：
4.8
通讯作者：
Brian J. Morris
中科院分区：
文献类型：
--
作者：
Daniel F. Catanzaro;N. Mesterovic;Brian J. Morris
通过测量肾素信使RNA（mRNA）（纳克/g组织）并与肾素活性（微摩尔血管紧张素I/h/g组织）进行比较，检查了小鼠肾脏和下颌下腺（SMG）中肾素基因的转录活性。对照组成年小鼠的肾素mRNA和肾素活性（括号内）为1.8 +/- 0.24（11 +/- 1.1）雄性肾脏，3.6 +/- 0.66（18 +/- 2.8），雄性SMG 230 +/- 34（903 +/- 59），雌性SMG 31 +/- 6（188 +/- 47）（平均值+/- SE，n = 6）。四种组织中肾素mRNA的表达比例与其活性的比例相似（1：2：100：17）。虽然雄性肾脏中的值是SMG中的百分之一，但每克组织涉及1000至10000倍的SMG细胞，因此，每个合成肾素的细胞，肾脏值将是SMG值的10至100倍。因此，肾肾小球细胞中的肾素基因表达可能高于SMG颗粒管细胞。成年男性SMG值是青春期时升高40倍的结果，去势后降至16%（+/- 3.8），但睾酮、T4、丙基硫氧嘧啶、钠耗竭或螺内酯给药未对SMG值产生显著影响。成年雌性SMG中的肾素mRNA比幼年值高18倍，睾酮使其增加10倍（+/- 1.6）（至成年雄性水平），T4使其增加5.5倍（+/- 0.81）（P <0.005），但丙基硫氧嘧啶使其降低至正常值的42%（+/- 29）（P <0.05）。丙基硫氧嘧啶导致睾酮给药的雌性值小幅但显著降低。1周后，肾脏中的肾素mRNA被卡托普利增加了3.0倍（+/- 0.30），被低钠饮食增加了2.3倍（+/- 0.23），被螺内酯增加了1.7倍（+/- 0.13），而T4、睾酮或丙基硫氧嘧啶几乎没有影响。唾液腺切除术增加了男性肾脏中的肾素mRNA和肾素，但在女性肾脏中没有，这表明SMG因子，可能是肾素，可能在抑制男性肾脏中的肾素合成中起作用。总之，测量的肾素mRNA表明，睾酮调节肾素基因表达的直接影响，在雄性小鼠SMG，而在女性的调节是由甲状腺激素。在肾脏中，增加肾素含量的条件伴随着肾素mRNA的平行（高5倍）增加，这表明在慢性低钠状态下肾肾小球细胞中的肾素基因表达增强。
The transcriptional activity of renin genes in the mouse kidney and submandibular gland (SMG) was examined by measurement of renin messenger RNA (mRNA) (nanograms per g tissue) and compared with renin activity (micromoles angiotensin I per h/g tissue). In control adult mice renin mRNA and renin activity (in parentheses) were 1.8 +/- 0.24 (11 +/- 1.1) in male kidney, 3.6 +/- 0.66 (18 +/- 2.8) in female kidney, 230 +/- 34 (903 +/- 59) in male SMG, and 31 +/- 6 (188 +/- 47) in female SMG (mean +/- SE, n = 6). The ratio of renin mRNA among these four tissues was similar to that of renin activity (1:2:100:17, respectively). Although the values in male kidney were one one-hundredth those in SMG, 1000 to 10000 times more SMG cells are involved per gram of tissue so that, per renin-synthesizing cell, kidney values would be 10 to 100 times SMG values. Expression of renin gene(s) in a renal juxtaglomerular cell may thus be higher than in a SMG granular duct cell. Values in adult male SMG were a consequence of a 40-fold rise at puberty, were decreased to 16% (+/- 3.8) by castration, but were not significantly influenced by treatment with testosterone, T4, propylthiouracil, sodium depletion, or spironolactone. Renin mRNA in adult female SMG was 18-fold higher than juvenile values, was increased 10-fold (+/- 1.6) by testosterone (to adult male levels) and 5.5-fold (+/- 0.81) by T4 (P less than 0.005), but was decreased to 42% (+/- 29) of normal by propylthiouracil (P less than 0.05). Propylthiouracil caused a small but significant decrease in testosterone-treated female values. In the kidney renin mRNA was increased 3.0-fold (+/- 0.30) by captopril, 2.3-fold (+/- 0.23) by a low sodium diet, and 1.7-fold (+/- 0.13) by spironolactone after 1 week, whereas T4, testosterone, or propylthiouracil had little or no effect. Sialoadenectomy increased renin mRNA and renin in male but not in female kidney, suggesting that a SMG factor, possibly renin, may have a role in suppression of renin synthesis in male kidney. In conclusion, measurement of renin mRNA suggests that testosterone regulates renin gene expression by a direct effect in male mouse SMG, whereas in the female regulation is by thyroid hormone. In the kidney conditions which increase renin content are accompanied by parallel (5-fold higher) increases in renin mRNA, suggesting enhanced expression of renin gene(s) in renal juxtaglomerular cells in chronic low sodium states.
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DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
White,BA;Bancroft,FC
通讯作者：
Bancroft,FC
小鼠颌下腺肾素和激肽释放酶的遗传和内分泌控制的比较。
影响因子：
6.1
作者：
Wilson,CM;Reynolds,RC;Ward,PE
通讯作者：
Ward,PE
小鼠颌下腺肾素热稳定性的遗传调控。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wilson,CM;Taylor,BA
通讯作者：
Taylor,BA
血管紧张素 II 免疫反应性与肾素共存于肾脏的肾小球旁颗粒细胞中。
DOI：
10.1073/pnas.78.6.3897
发表时间：
1981
影响因子：
11.1
作者：
Celio,MR;Inagami,T
通讯作者：
Inagami,T
NaI 中固定在硝化纤维素上的 mRNA 的生物活性。
DOI：
10.1073/pnas.80.21.6523
发表时间：
1983
影响因子：
11.1
作者：
Bresser,J;Hubbell,HR;Gillespie,D
通讯作者：
Gillespie,D