Modeling the dynamics of a non-limited and a self-limited gene drive system in structured Aedes aegypti populations.
Modeling the dynamics of a non-limited and a self-limited gene drive system in structured Aedes aegypti populations.
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DOI：
10.1371/journal.pone.0083354
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gould F
中科院分区：
文献类型：
--
作者：
Legros M;Xu C;Morrison A;Scott TW;Lloyd AL;Gould F
最近，在控制传播疾病的昆虫种群的遗传策略方面取得了重大进展。其中一些策略利用自私遗传元件的基因驱动特性，将物理上相连的抗病原体基因传播到当地的媒介种群中。由于这些自私因素有可能在人群中传播，因此必须仔细评估基于这些策略的控制方法，以确保在导致难以控制的遗传货物的可取传播与避免传播到非目标人群等潜在有害结果之间取得平衡。还需要对这类释放的经济进行更好的估计。在这里，我们使用一个生物现实的数学模型来模拟秘鲁伊基托斯的埃及伊蚊种群，对两种这样的策略进行评估。一种策略使用自私元素美狄亚，这是一种可以永久传播到大片地理区域的无限元素；另一种策略依赖于杀手-救援基因结构，并被预测为有限的空间和时间传播。我们模拟了部署这些遗传策略的各种操作方法，并量化了实现Medea连锁基因和/或高频率Killer-Rescue相关元件的最终传播所需的最佳转基因蚊子数量。我们表明，对于这两种策略，在三年内实现抗病原体基因传播的最有效的方法通常是随着时间的推移分多次释放两性成虫。尽管这些释放中的雌性不应该传播疾病，但公众可能会对这种释放感到担忧，这使得效率较低的只释放男性的释放更实用。这项研究为种群替换遗传策略的操作方法提供了指导方针，并说明了使用详细的空间模型来帮助安全和有效地实施这种新的遗传策略。
Recently there have been significant advances in research on genetic strategies to control populations of disease-vectoring insects. Some of these strategies use the gene drive properties of selfish genetic elements to spread physically linked anti-pathogen genes into local vector populations. Because of the potential of these selfish elements to spread through populations, control approaches based on these strategies must be carefully evaluated to ensure a balance between the desirable spread of the refractoriness-conferring genetic cargo and the avoidance of potentially unwanted outcomes such as spread to non-target populations. There is also a need to develop better estimates of the economics of such releases. We present here an evaluation of two such strategies using a biologically realistic mathematical model that simulates the resident Aedes aegypti mosquito population of Iquitos, Peru. One strategy uses the selfish element Medea, a non-limited element that could permanently spread over a large geographic area; the other strategy relies on Killer-Rescue genetic constructs, and has been predicted to have limited spatial and temporal spread. We simulate various operational approaches for deploying these genetic strategies, and quantify the optimal number of released transgenic mosquitoes needed to achieve definitive spread of Medea-linked genes and/or high frequencies of Killer-Rescue-associated elements. We show that for both strategies the most efficient approach for achieving spread of anti-pathogen genes within three years is generally to release adults of both sexes in multiple releases over time. Even though females in these releases should not transmit disease, there could be public concern over such releases, making the less efficient male-only release more practical. This study provides guidelines for operational approaches to population replacement genetic strategies, as well as illustrates the use of detailed spatial models to assist in safe and efficient implementation of such novel genetic strategies.
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影响因子：
2
作者：
Davis, S;Bax, N;Grewe, P
通讯作者：
Grewe, P
影响因子：
--
作者：
Gould, F;Magori, K;Huang, YX
通讯作者：
Huang, YX
DOI：
10.1098/rspb.2008.0846
发表时间：
2008-12-22
影响因子：
4.7
作者：
Gould, Fred;Huang, Yunxin;Lloyd, Alun L.
通讯作者：
Lloyd, Alun L.
影响因子：
2.1
作者：
Mori, A;Chadee, DD;Severson, DW
通讯作者：
Severson, DW
影响因子：
56.9
作者：
Chen, Chun-Hong;Huang, Haixia;Hay, Bruce A.
通讯作者：
Hay, Bruce A.