Transcription regulation of sex-biased genes during ontogeny in the malaria vector Anopheles gambiae.
Transcription regulation of sex-biased genes during ontogeny in the malaria vector Anopheles gambiae.
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DOI：
10.1371/journal.pone.0021572
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Crisanti A
中科院分区：
文献类型：
--
作者：
Magnusson K;Mendes AM;Windbichler N;Papathanos PA;Nolan T;Dottorini T;Rizzi E;Christophides GK;Crisanti A
在冈比亚按蚊中，性别调节基因负责控制性别二型性，因此在确定雌性蚊子传播人类疟疾的能力方面至关重要。这些基因的鉴定和功能特征将揭示蚊子的性发育和成熟，并为旨在降低蚊子生育力和/或扭曲性别比例的遗传控制措施提供有用的目标。我们对从发育早期到成年期的性别调控基因进行了全基因组转录分析，并对新的性腺基因进行了功能筛选。我们的结果表明，偏向男性的基因经历了从幼虫期到成年期的主要转录转换。与基因组的其余部分相比，成年阶段的男性偏向基因包括大量独特的序列。这与偏向女性的基因形成对比，后者更加保守，主要在发育后期被激活。独特序列的高频率将表明，偏向男性的基因比基因组的其他部分进化得更快。这一发现特别耐人寻味，因为冈比亚按蚊是一种严格意义上的雌性一夫一妻制物种，这表明除了精子竞争之外，驱动力必须解释偏向男性的基因的快速进化。我们还鉴定了一些以前未知的冈比亚按蚊睾丸和卵巢特异基因，并对其进行了功能鉴定。其中两个基因，组蛋白去乙酰酶共抑制复合体的零种群增长和缺陷沉默3区的抑制因子，被证明在性腺发育中起关键作用。
In Anopheles gambiae, sex-regulated genes are responsible for controlling gender dimorphism and are therefore crucial in determining the ability of female mosquitoes to transmit human malaria. The identification and functional characterization of these genes will shed light on the sexual development and maturation of mosquitoes and provide useful targets for genetic control measures aimed at reducing mosquito fertility and/or distorting the sex ratio. We conducted a genome wide transcriptional analysis of sex-regulated genes from early developmental stages through adulthood combined with functional screening of novel gonadal genes. Our results demonstrate that the male-biased genes undergo a major transcription turnover starting from larval stages to adulthood. The male biased genes at the adult stage include a significant high number of unique sequences compared to the rest of the genome. This is in contrast to female-biased genes that are much more conserved and are mainly activated during late developmental stages. The high frequency of unique sequences would indicate that male-biased genes evolve more rapidly than the rest of the genome. This finding is particularly intriguing because A. gambiae is a strictly female monogamous species suggesting that driving forces in addition to sperm competition must account for the rapid evolution of male-biased genes. We have also identified and functionally characterized a number of previously unknown A. gambiae testis- and ovary-specific genes. Two of these genes, zero population growth and a suppressor of defective silencing 3 domain of the histone deacetylase co-repressor complex, were shown to play a key role in gonad development.
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影响因子：
12.3
作者：
Parisi M;Nuttall R;Edwards P;Minor J;Naiman D;Lü J;Doctolero M;Vainer M;Chan C;Malley J;Eastman S;Oliver B
通讯作者：
Oliver B
影响因子：
3
作者：
Howell PI;Knols BG
通讯作者：
Knols BG
影响因子：
5.3
作者：
Alland, L;David, G;DePinho, RA
通讯作者：
DePinho, RA
影响因子：
2.4
作者：
Horn, C;Wimmer, EA
通讯作者：
Wimmer, EA
DOI：
10.1073/pnas.092274999
发表时间：
2002-06-25
影响因子：
11.1
作者：
Dimopoulos, G;Christophides, GK;Kafatos, FC
通讯作者：
Kafatos, FC