Organ-Chips Enhance the Maturation of Human iPSC-Derived Dopamine Neurons.
Organ-Chips Enhance the Maturation of Human iPSC-Derived Dopamine Neurons.
复制标题
器官芯片增强人iPSC衍生的多巴胺神经元的成熟。
DOI：
10.3390/ijms241814227
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发表时间：
2023-09-18
影响因子：
5.6
通讯作者：
Svendsen CN
中科院分区：
文献类型：
--
作者：
Otero MG;Bell S;Laperle AH;Lawless G;Myers Z;Castro MA;Villalba JM;Svendsen CN
虽然人体中的细胞在血液供应不断提供营养并去除废物的环境中发挥作用，但常规组织培养孔平台中的细胞在其上方的静态培养基池中生长，并且通常缺乏成熟度，限制了它们在健康和疾病中研究细胞生物学的效用。相比之下，器官芯片微流体系统允许细胞在恒定流动下生长，更类似于体内情况。在这里，我们将人类诱导多能干细胞分化为多巴胺神经元，并在传统的多孔培养物和器官芯片中评估细胞特性。我们表明，器官芯片文化，多孔文化相比，提供了一个整体的更大比例和同质性的多巴胺能神经元以及成熟标志物的水平增加。这些器官芯片是研究成熟多巴胺神经元的理想平台，可以更好地了解它们在健康和最终神经系统疾病中的生物学特性。
While cells in the human body function in an environment where the blood supply constantly delivers nutrients and removes waste, cells in conventional tissue culture well platforms are grown with a static pool of media above them and often lack maturity, limiting their utility to study cell biology in health and disease. In contrast, organ-chip microfluidic systems allow the growth of cells under constant flow, more akin to the in vivo situation. Here, we differentiated human induced pluripotent stem cells into dopamine neurons and assessed cellular properties in conventional multi-well cultures and organ-chips. We show that organ-chip cultures, compared to multi-well cultures, provide an overall greater proportion and homogeneity of dopaminergic neurons as well as increased levels of maturation markers. These organ-chips are an ideal platform to study mature dopamine neurons to better understand their biology in health and ultimately in neurological disorders.
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影响因子：
64.8
作者：
Kriks, Sonja;Shim, Jae-Won;Piao, Jinghua;Ganat, Yosif M.;Wakeman, Dustin R.;Xie, Zhong;Carrillo-Reid, Luis;Auyeung, Gordon;Antonacci, Chris;Buch, Amanda;Yang, Lichuan;Beal, M. Flint;Surmeier, D. James;Kordower, Jeffrey H.;Tabar, Viviane;Studer, Lorenz
通讯作者：
Studer, Lorenz
影响因子：
64.5
作者：
Park IH;Arora N;Huo H;Maherali N;Ahfeldt T;Shimamura A;Lensch MW;Cowan C;Hochedlinger K;Daley GQ
通讯作者：
Daley GQ
影响因子：
8.8
作者：
Lim RG;Quan C;Reyes-Ortiz AM;Lutz SE;Kedaigle AJ;Gipson TA;Wu J;Vatine GD;Stocksdale J;Casale MS;Svendsen CN;Fraenkel E;Housman DE;Agalliu D;Thompson LM
通讯作者：
Thompson LM
影响因子：
15.9
作者：
Bose, Anindita;Petsko, Gregory A.;Studer, Lorenz
通讯作者：
Studer, Lorenz
影响因子：
25
作者：
Kamath, Tushar;Abdulraouf, Abdulraouf;Burris, S. J.;Langlieb, Jonah;Gazestani, Vahid;Nadaf, Naeem M.;Balderrama, Karol;Vanderburg, Charles;Macosko, Evan Z.
通讯作者：
Macosko, Evan Z.