Changes in median eminence of fatty acid-binding protein 3 in a mouse model of pain.
Changes in median eminence of fatty acid-binding protein 3 in a mouse model of pain.
复制标题
DOI：
10.1002/npr2.12225
复制
发表时间：
2022-03
影响因子：
2.5
通讯作者：
Tokuyama S
中科院分区：
文献类型：
--
作者：
Tachibana D;Nakamoto K;Tokuyama S
脂肪酸结合蛋白（FABP）调节多不饱和脂肪酸（PUFA）的细胞内运输和信号转导。我们前期的研究表明，下丘脑PUFA的变化与疼痛过程有关。然而，FABP亚型在疼痛过程中如何变化仍不清楚。在此，我们研究了术后疼痛模型小鼠下丘脑FABP亚型的表达变化和定位。采用爪切口诱导的术后方法作为雄性ddY小鼠的疼痛模型。使用von Frey试验检查机械性异常性疼痛。通过真实的时间PCR测量几种FABP mRNA的分析，并通过免疫荧光研究测量其蛋白水平的细胞定位。术后疼痛小鼠于术后第2天出现机械性痛觉超敏，术后疼痛小鼠下丘脑FABP 3 mRNA表达较对照组显著增加。FABP 3蛋白在正中隆起和弓状核中表达，并与小胶质细胞标志物Iba-1共定位。其蛋白水平在切创后第2天在正中隆起显著升高，并在切创后第4天恢复至对照水平。我们的研究结果表明，FABP 3在正中隆起可能会改变疼痛刺激，并可能代表一个分子联系控制疼痛。在正中隆起，FABP 3蛋白表达水平在切爪后第2天与对照组相比显著增加，并在切爪后第4天恢复到对照水平。
Fatty acid–binding protein (FABP) regulates polyunsaturated fatty acid (PUFA) intracellular trafficking and signal transduction. Our previous studies demonstrated that the alteration of PUFA in the hypothalamus is involved in pain process. However, how FABP subtypes change during pain remain unclear. Here, we examined the expression changes and localization in the hypothalamic FABP subtype in postoperative pain model mice. Paw incision–induced postoperative methods were adopted as a pain model in male ddY mice. Mechanical allodynia was examined using the von Frey test. The analysis of several FABPs mRNA was measured by real‐time PCR, and cellular localization of its protein level was measured by immunofluorescent study. Postoperative pain mouse elicited mechanical allodynia on Day 2 after paw incision, and mRNA expression of FABP3 increased significantly in the hypothalamus in the postoperative pain mouse model compared to that in control mice. FABP3 protein expressed in the median eminence and the arcuate nucleus, and colocalized with Iba‐1, which is a microglial cell marker. Its protein level significantly increased in the median eminence on Day 2 after incision and returned to the control level on Day 4 after incision. Our findings indicate that FABP3 in the median eminence may change in pain stimuli and may represent a molecular link controlling pain. In the median eminence, the protein expression of FABP3 levels was significantly increased on Day 2 after the paw incision compared with Control group, and returned to the control level on Day 4 after paw incision.
登录
查看更多内容
影响因子：
2.8
作者：
Owada, Y;Yoshimoto, T;Kondo, H
通讯作者：
Kondo, H
影响因子：
3.3
作者：
Kaczocha M;Glaser ST;Maher T;Clavin B;Hamilton J;O'Rourke J;Rebecchi M;Puopolo M;Owada Y;Thanos PK
通讯作者：
Thanos PK
影响因子：
6.2
作者：
Hofmann, Kristina;Lamberz, Christian;Kuerschner, Lars
通讯作者：
Kuerschner, Lars
影响因子：
15.9
作者：
Patwardhan, Amol M.;Akopian, Armen N.;Hargreaves, Kenneth M.
通讯作者：
Hargreaves, Kenneth M.
影响因子：
56.9
作者：
COX, VC;VALENSTEIN, ES
通讯作者：
VALENSTEIN, ES