Molybdenum disulfide/graphdiyne-based photoactive material derived photoelectrochemical strategy for highly sensitive MicroRNA assay
Molybdenum disulfide/graphdiyne-based photoactive material derived photoelectrochemical strategy for highly sensitive MicroRNA assay
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基于二硫化钼/石墨二炔的光活性材料衍生的光电化学策略，用于高灵敏度的 MicroRNA 测定
DOI：
10.1016/j.snb.2019.126808
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发表时间：
2019-10
期刊：
影响因子：
--
通讯作者：
Hun Xu
中科院分区：
文献类型：
--
作者：
Li Xiaohua;Li Yanxin;Zhang Jianying;Meng Yuchan;Yu Xijuan;Wang Xiao;Hun Xu
本研究成功制备了二硫化钼-石墨二炔（MoS 2-GDY）。利用GDY的固有还原性，将（NH_4）_2MoS_4还原成MoS_2，形成MoS_2-GDY复合光活性物质。MoS_2-GDY利用了MoS_2的大禁带宽度和GDY的窄禁带宽度。GDY的自然窄带隙增强了光的吸收，与MoS 2的结合降低了电子-空穴复合速率，从而获得了较强的光电化学（PEC）信号。MoS 2-GDY的PEC信号随GDY与（NH 4）2 MoS 4的质量比而变化。MoS 2-GDY在1：25产生最强的PEC性能。以1：25的MoS 2-GDY质量比制备检测microRNA-141的PEC检测试剂。为了获得低背景噪声和强PEC信号，设计了一种双修饰探针耦合生物条形码AuNPs和杂交链反应策略。双修饰探针一侧标记MoS 2-GDY，另一侧标记生物素。PEC的强度依赖于microRNA-141的浓度。在pH7.5，电位0.2 V的条件下，该方法的线性范围为1.0 × 10 - 17 ~ 1.0 × 10- 9 mol/L，检出限为3.3 × 10- 18 mol/L。该方法已成功应用于复杂生物基质中microRNA-141的测定。
In this study, molybdenum disulfide-graphdiyne (MoS2-GDY) was successfully prepared. Utilizing the inherent reducibility of GDY, (NH4)2MoS4was reduced to MoS2forming photoactive material MoS2-GDY complex. MoS2-GDY made use of the large band-gap of MoS2and the narrow band-gap of GDY. The naturally narrow band-gap of GDY enhanced the absorption of light and the combination of GDY and MoS2reduced the electron-hole recombination rate so as to gain strong photoelectrochemical (PEC) signal. The PEC signal of MoS2-GDY changed with the mass ratio of GDY to (NH4)2MoS4. MoS2-GDY produced the strongest PEC performance at 1:25. MoS2-GDY with the mass ratio of 1:25 was used to fabricate a PEC assay for microRNA-141 detection. To obtain low background noise and strong PEC signal, a dual-modified probe coupling bio-bar code AuNPs and hybridization chain reaction strategy was designed. The dual-modified probe which labeled with MoS2-GDY on the one side, and marked with biotin on the other side. And the intensity of PEC depended on the concentration of microRNA-141. Under optimal conditions, 0.2 V potential, pH 7.5, the PEC assay had a linear range in 1.0 × 10−17mol/L to 1.0 × 10-9mol/L and a low detection limit of 3.3 × 10-18mol/L. The assay has been successfully applied to the determination of microRNA-141 in complex biological matrixes.
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影响因子：
12.6
作者：
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通讯作者：
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DOI：
10.2147/nsa
发表时间：
--
期刊：
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影响因子：
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影响因子：
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作者：
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影响因子：
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