Transcription Regulator YgeK Affects the Virulence of Avian Pathogenic Escherichia coli.
Transcription Regulator YgeK Affects the Virulence of Avian Pathogenic Escherichia coli.
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转录调节因子 YgeK 影响禽致病性大肠杆菌的毒力。
DOI：
10.3390/ani11113018
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发表时间：
2021-10-20
期刊：
影响因子：
--
通讯作者：
Qi K
中科院分区：
文献类型：
--
作者：
Tu J;Fu D;Gu Y;Shao Y;Song X;Xue M;Qi K
禽致病性大肠杆菌 (APEC) 是家禽大肠杆菌病的主要病原体。转录调节因子YgeK是APEC中位于大肠杆菌三型分泌系统2（ETT2）的转录调节因子。但YgeK在APEC中的作用尚未见报道。在本研究中，我们发现APEC中YgeK的失活降低了鞭毛形成能力、细菌运动能力、血清敏感性、粘附能力和毒力。结果表明转录调节因子 YgeK 在 APEC 毒力中发挥着至关重要的作用。禽致病性大肠埃希菌（APEC）是家禽大肠杆菌病的主要病原体，也是人类肠外致病性大肠埃希菌的潜在基因来源。 大肠杆菌 III 型分泌系统 2 (ETT2) 广泛分布于人和动物 ExPEC 分离株中，对 ExPEC 的毒力至关重要。转录调节因子 YgeK 位于 ETT2 基因簇中，被确定为肠出血性大肠杆菌 (EHEC) 基因表达的重要调节因子。但YgeK在APEC中的作用尚未见报道。在本研究中，我们对不同大肠杆菌菌株之间的YgeK进行了氨基酸比对分析，并从临床APEC菌株AE81中产生了ygeK突变菌株AE81ΔygeK。 APEC 中 YgeK 失活后，鞭毛形成、细菌运动、血清敏感性、粘附和毒力均显着降低。然后，我们进行转录组测序来分析生物过程中涉及的功能途径。结果表明，ETT2 转录调节因子 YgeK 在 APEC 毒力中发挥着至关重要的作用。这些发现有助于我们理解ETT2簇的功能，并阐明APEC的致病机制。
Avian pathogenic Escherichia coli (APEC) is the responsible pathogen for colibacillosis in poultry. Transcriptional regulator YgeK was a transcriptional regulator locating at E. coli type three secretion system 2 (ETT2) in APEC. However, the role of YgeK in APEC has not been reported. In this study, we found that the inactivation of YgeK in APEC decreased the flagellar formation ability, bacterial motility ability, serum sensitivity, adhesion ability, and virulence. Results suggested that the transcriptional regulator YgeK plays a crucial role in APEC virulence. Avian pathogenic Escherichia coli (APEC) is the responsible pathogen for colibacillosis in poultry, and is a potential gene source for human extraintestinal pathogenic Escherichia coli. Escherichia coli type III secretion system 2 (ETT2) is widely distributed in human and animal ExPEC isolates, and is crucial for the virulence of ExPEC. Transcriptional regulator YgeK, located in the ETT2 gene cluster, was identified as an important regulator of gene expression in enterohemorrhagic E. coli (EHEC). However, the role of YgeK in APEC has not been reported. In this study, we performed amino acid alignment analysis of YgeK among different E. coli strains and generated ygeK mutant strain AE81ΔygeK from clinical APEC strain AE81. Flagellar formation, bacterial motility, serum sensitivity, adhesion, and virulence were all significantly reduced following the inactivation of YgeK in APEC. Then, we performed transcriptome sequencing to analyze the functional pathways involved in the biological processes. Results suggested that ETT2 transcriptional regulator YgeK plays a crucial role in APEC virulence. These findings thus contribute to our understanding of the function of the ETT2 cluster, and clarify the pathogenic mechanism of APEC.
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影响因子：
3.7
作者：
Green ER;Mecsas J
通讯作者：
Mecsas J
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
3.1
作者：
Herren, Christopher D.;Mitra, Arindam;Mukhopadhyay, Suman
通讯作者：
Mukhopadhyay, Suman
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
9.4
作者：
Makino, SI;Tobe, T;Sasakawa, C
通讯作者：
Sasakawa, C