Chinese medicinal formula, Qinggan Huoxue Recipe protects rats from alcoholic liver disease via the lipopolysaccharide-Kupffer cell signal conduction pathway.
Chinese medicinal formula, Qinggan Huoxue Recipe protects rats from alcoholic liver disease via the lipopolysaccharide-Kupffer cell signal conduction pathway.
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中药清肝活血方通过脂多糖-枯否细胞信号传导通路保护大鼠酒精性肝病
DOI：
10.3892/etm.2014.1740
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发表时间：
2014-08
影响因子：
2.7
通讯作者：
Ji G
中科院分区：
文献类型：
--
作者：
Wu T;Liu T;Zhang L;Xing LJ;Zheng PY;Ji G
中药复方清肝活血方具有一系列药理作用，包括可逆性脂肪变性、降低炎性细胞因子水平和抗脂质过氧化作用。本研究的目的是通过脂多糖-枯否细胞（LPS-KC）信号传导通路，探讨清肝泻心方对酒精性肝病（ALD）大鼠肝脏保护作用的具体机制。采用QGR和HXR复合因子对ALD大鼠进行干预。采用苏木精-伊红染色法评价清气活血方治疗后肝脏的病理变化，采用酶联免疫吸附法测定血浆中肿瘤坏死因子（TNF）-α的含量。免疫组织化学染色检测细胞分化抗原（CD）68和14的表达。Western blot和逆转录-聚合酶链反应检测Toll样受体4（TLR 4）、磷酸化细胞外调节蛋白激酶（p-ERK）、核因子（NF）-κB、CD 14和TNF-α的表达。在复合因子刺激后，大鼠表现出丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）水平升高，以及明显的病理变化。此外，LPS-KC信号传导通路中的相关分子被上调，并且QGHXR被鉴定为在ALD大鼠的LPS-KC信号传导通路中有效。清肝活血方在降低血清ALT、AST水平，调节CD 14、TLR 4、NF-κB、ERK、TNF-α水平，改善病理改变方面上级清肝冲剂和活血方。提示清气活血方可能通过调节LPS-KC信号通路中的相关分子来治疗ALD。
The Chinese medicinal formula, Qinggan (QG) Huoxue (HX) Recipe (R) exerts a range of pharmacological effects, including reversible steatosis, decreased levels of inflammatory cytokines and lipid peroxidation resistance. The aim of the present study was to determine the specific mechanisms of QGHXR hepatoprotection through the lipopolysaccharide-Kupffer cell (LPS-KC) signal conduction pathway in rats with alcoholic liver disease (ALD). ALD rats were exposed to the compound factors, QGR and HXR. Hematoxylin and eosin staining was conducted to evaluate the pathological changes in the liver following QGHXR treatment and an enzyme-linked immunosorbent assay was performed to measure the content of tumor necrosis factor (TNF)-α in the plasma. Immunohistochemical staining was conducted to examine the expression of cell differentiation antigen (CD) 68 and 14. In addition, western blot analysis and reverse transcription-polymerase chain reaction were used to measure the expression of Toll-like receptor 4 (TLR4), phosphorylated-extracellular regulated protein kinases (p-ERK), nuclear factor (NF)-κB, CD14 and TNF-α. Following stimulation with the compound factors, the rats exhibited increased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, as well as marked pathological changes. Furthermore, the related molecules in the LPS-KC pathway were upregulated and QGHXR was identified to be effective in the LPS-KC signal conduction pathway in the ALD rats. QGHXR was superior to QGR and HXR in reducing the serum ALT and AST levels, regulating CD14, TLR4, NF-κB, ERK and TNF-α as well as improving the pathological changes. The results indicated that QGHXR therapy may provide a novel strategy for treating ALD via regulation of the related molecules in the LPS-KC signaling pathway.
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DOI：
10.1111/j.1530-0277.2008.00827.x
发表时间：
2009-02
期刊：
ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH
影响因子：
--
作者：
Purohit, Vishnudutt;Gao, Bin;Song, Byoung-Joon
通讯作者：
Song, Byoung-Joon
DOI：
10.1152/ajpgi.00094.2004
发表时间：
2004-08-01
影响因子：
4.5
作者：
Hines, IN;Wheeler, MD
通讯作者：
Wheeler, MD
影响因子：
4.4
作者：
Ghosh, S
通讯作者：
Ghosh, S
DOI：
10.1084/jem.175.6.1697
发表时间：
1992-06-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Lee JD;Kato K;Tobias PS;Kirkland TN;Ulevitch RJ
通讯作者：
Ulevitch RJ
影响因子：
4.4
作者：
Uesugi, T;Froh, M;Thurman, RG
通讯作者：
Thurman, RG