Characteristics of polygalacturonate lyase C from Bacillus subtilis 7-3-3 and its synergistic action with PelA in enzymatic degumming.
Characteristics of polygalacturonate lyase C from Bacillus subtilis 7-3-3 and its synergistic action with PelA in enzymatic degumming.
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枯草芽孢杆菌7-3-3聚半乳糖醛酸裂解酶C的特性及其与PelA酶法脱胶的协同作用
DOI：
10.1371/journal.pone.0079357
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Qu Y
中科院分区：
文献类型：
--
作者：
Zou M;Li X;Zhao J;Qu Y
从枯草芽孢杆菌7-3-3菌株PELC中发现了一种碱性多聚半乳糖醛酸裂解酶(PGL)，该酶对不同的果胶底物具有不同的解聚能力。PELC的PGL活性随底物甲酯化程度的增加而降低。PELA和PELC在苎麻纤维酶法脱胶中表现出显著的协同效应。当PELC取代高达PELA剂量的八分之三(PELC，60U g−1苎麻纤维)时，牙胶损失率增加了62%。据我们所知，这项研究首次报道了以PELA和PELC为代表的多糖裂解酶家族1和3成员的协同作用。为酶法脱胶用酶的改进和生产提供了新的思路。
An alkaline polygalacturonate lyase (PGL) from Bacillus subtilis 7-3-3, PelC, with diverse depolymerization abilities for different pectin substrates was found. The PGL activity of PelC decreased with increasing degree of methyl esterification of the substrate. PelA and PelC displayed notable synergistic effects in the enzymatic degumming of ramie fibers. Gum loss rates increased by 62% when PelC was used to replace up to three-eighths of the PelA dose (PelC, 60 U g−1 ramie fibers). To the best of our knowledge, this study is the first to report the synergistic action of members of polysaccharide lyase families 1 and 3, represented by PelA and PelC, respectively. The present paper provides new insights into the improvement and production of enzymes used in enzymatic degumming.
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影响因子：
--
作者：
Guo F;Zou M;Li X;Zhao J;Qu Y
通讯作者：
Qu Y
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
DOI：
10.1007/s10295-008-0490-y
发表时间：
2009-02-01
影响因子：
3.4
作者：
Basu, Snehasish;Saha, Manabendra N.;Chakrabarti, Krishanu
通讯作者：
Chakrabarti, Krishanu
DOI：
10.1107/s0907444901014482
发表时间：
2001-12-01
影响因子：
2.2
作者：
Akita, M;Suzuki, A;Yamane, T
通讯作者：
Yamane, T