An effective degumming enzyme from Bacillus sp. Y1 and synergistic action of hydrogen peroxide and protease on enzymatic degumming of ramie fibers.
An effective degumming enzyme from Bacillus sp. Y1 and synergistic action of hydrogen peroxide and protease on enzymatic degumming of ramie fibers.
复制标题
DOI：
10.1155/2013/212315
复制
发表时间：
2013
影响因子：
--
通讯作者：
Qu Y
中科院分区：
文献类型：
--
作者：
Guo F;Zou M;Li X;Zhao J;Qu Y
酶法脱胶作为一种替代化学脱胶的方法受到了广泛的关注。然而，迄今为止，除果胶酶外，其他酶组分对脱胶效果的研究报道甚少，漂白剂（H2 O2）对酶法脱胶效果的影响以及酶法脱胶与H2 O2漂白联合工艺的研究未见报道。本研究发现野生型芽孢杆菌Y1的粗酶具有强大而快速的脱胶能力。PGL活性在pH9.6 -10.0和60°C时最高，在pH 7-10.5和30-50°C时稳定，pH和温度范围较宽。PGL对H_2O_2的耐受性也很好，加入H_2O_2进行脱胶可显著改善纤维的失胶率和白度。它与H_2O_2对苎麻纤维的脱胶漂白也有协同作用。结果表明，该工艺非常适合酶法脱胶和H2 O2漂白联合工艺。与对照果胶酶相比，它还含有更多的蛋白质，并且其高蛋白酶含量被进一步证实为有效脱胶的因素。蛋白酶和果胶酶对脱胶也有协同作用。
Enzymatic degumming, as an alternative to chemical processing, has attracted wide attention. However, to date, little information about other enzyme components with effective degumming except pectinase has been reported, and there is no report about the effect of bleaching agent (H2O2) on enzymatic degumming and combining enzymatic degumming and H2O2 bleaching process. In this study, we found that the crude enzyme of wild-type Bacillus sp. Y1 had a powerful and fast degumming ability. Its PGL activity was the highest at pH 9.6–10.0 and 60°C and stable at pH 7–10.5 and 30–50°C, having a wide scope of pH and temperature. Its PGL also had a high H2O2 tolerance, and the gum loss and brightness of fibers could be significantly improved when H2O2 was added into it for degumming. The synergistic action was also found between it and H2O2 on the degumming and bleaching of ramie fibers. All showed that it was very suitable for a joint process of enzymatic degumming and H2O2 bleaching. It also contained more proteins compared with a control pectinase, and its high protease content was further substantiated as a factor for effective degumming. Protease and pectinase also had a synergistic action on degumming.
登录
查看更多内容
影响因子：
5.7
作者：
Brooks, RE;Moore, SB
通讯作者：
Moore, SB
影响因子：
5.7
作者：
Chen, Jing;Yi, Jie;Liu, Zhong-Wen
通讯作者：
Liu, Zhong-Wen
影响因子：
1.6
作者：
Kobayashi, T;Koike, K;Ito, S
通讯作者：
Ito, S
DLBS1033是lumbricus rubellus的蛋白质提取物，具有抗血栓形成和溶栓活性。
影响因子：
--
作者：
Trisina J;Sunardi F;Suhartono MT;Tjandrawinata RR
通讯作者：
Tjandrawinata RR
影响因子：
2.8
作者：
Nakkeeran, Ekambaram;Gowthaman, Marichetti Kuppuswamy;Subramanian, Rangaswamy
通讯作者：
Subramanian, Rangaswamy