Dynamics of interferon-gamma release assay and cytokine profiles in blood and respiratory tract specimens from mice with tuberculosis and the effect of therapy.
Dynamics of interferon-gamma release assay and cytokine profiles in blood and respiratory tract specimens from mice with tuberculosis and the effect of therapy.
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DOI：
10.1007/s10096-011-1428-2
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发表时间：
2012-06
影响因子：
4.5
通讯作者：
Bakker-Woudenberg, I. A. J. M.
中科院分区：
文献类型：
--
作者：
de Steenwinkel, J. E. M.;de Knegt, G. J.;ten Kate, M. T.;Verbrugh, H. A.;Ottenhoff, T. H. M.;Bakker-Woudenberg, I. A. J. M.
区分活动性和潜伏性结核病（TB）的诊断方法存在局限性，并且预测潜伏进展为TB疾病仍然很复杂。传统上，结核病特异性宿主免疫反应是可视化使用结核菌素皮肤试验。目前，IFN-γ释放试验（IGRA）提供了一种更特异、更灵敏的检测结核分枝杆菌暴露的方法。然而，IGRA辅助诊断活动性结核病的优点尚不清楚。我们将IGRA用于小鼠，并使用基于微珠的流式细胞术技术定量评估未治疗感染过程中的细胞因子谱，并研究IGRA和细胞因子作为治疗反应生物标志物的价值。在活动性TB感染进展过程中，IGRA结果的高度变异性与感染的各个阶段有关。然而，观察到IGRA结果和IFN-γ、IL-17、IP-10或IFN-γ水平显著降低，似乎与成功治疗相关。该结果不支持IGRA在准确诊断活动性结核或监测感染进展方面的价值。然而，IGRA被证明是一个有用的生物标志物，以监测治疗成功。此外，不同的细胞因子可作为生物标志物。
There are limitations on diagnostic methods to differentiate between active and latent tuberculosis (TB), and the prediction of latent progression to TB disease is yet complex. Traditionally, tuberculosis-specific host immune response was visualized using the tuberculin skin test. Nowadays, IFN-γ release assays (IGRA) provide a more specific and sensitive tool, by which exposure to Mtb could be determined. However, the merit of IGRA aids in diagnosing active TB is yet unclear. We adapted IGRA for use in mice, and quantifying bead-based flow cytometry techniques were used to assess cytokine profiles during the course of untreated infection and to investigate the value of IGRA and cytokines as biomarkers for therapy response. High variability of IGRA results during progression of active TB infection related to various phases of infection was obtained. However, a significant decrease in IGRA results and in levels of IFN-γ, IL-17, IP-10 or MIG was observed and appeared to be associated with successful therapy. This outcome does not support the value of IGRA to accurately diagnose active TB or to monitor infection progression. However, IGRA proved to be a useful biomarker to monitor therapy success. In addition, different cytokines might serve as biomarkers.
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DOI：
10.1177/039463200902200318
发表时间：
2009-07-01
影响因子：
3.5
作者：
De Steenwinkel, J. E. M.;De Knegt, G. J.;Bakker-Woudenberg, I. A. J. M.
通讯作者：
Bakker-Woudenberg, I. A. J. M.
DOI：
10.1164/rccm.200904-0557oc
发表时间：
2009-10-01
影响因子：
24.7
作者：
Jafari, Claudia;Thijsen, Steven;Lange, Christoph
通讯作者：
Lange, Christoph
影响因子：
15.8
作者：
Rosenthal IM;Zhang M;Williams KN;Peloquin CA;Tyagi S;Vernon AA;Bishai WR;Chaisson RE;Grosset JH;Nuermberger EL
通讯作者：
Nuermberger EL
DOI：
10.1073/pnas.0702257104
发表时间：
2007-05-08
影响因子：
11.1
作者：
Joosten, Simone A.;van Meijgaarden, Krista E.;Ottenhoff, Tom H. M.
通讯作者：
Ottenhoff, Tom H. M.
DOI：
10.1084/jem.20091885
发表时间：
2010-07-05
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Shafiani S;Tucker-Heard G;Kariyone A;Takatsu K;Urdahl KB
通讯作者：
Urdahl KB