Distinct expression profiles of peptides in placentae from preeclampsia and normal pregnancies.
Distinct expression profiles of peptides in placentae from preeclampsia and normal pregnancies.
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先兆子痫和正常妊娠胎盘中肽的独特表达谱
DOI：
10.1038/s41598-020-74840-0
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发表时间：
2020-10-16
影响因子：
4.6
通讯作者：
Jia R
中科院分区：
文献类型：
--
作者：
Huang J;Ling Z;Zhong H;Yin Y;Qian Y;Gao M;Ding H;Cheng Q;Jia R
本研究试图从胎盘中寻找与子痫前期相关的潜在生物活性多肽，以获得预测、诊断和治疗子痫前期的信息。采用液-质联用技术，对正常妊娠和PE妊娠胎盘肽进行了比较分析。利用基因本体论(GO)、通径分析和独创性通径分析(IPA)，根据蛋白质前体对差异多肽的潜在生物学功能进行了评价。通过Transwell实验和定量聚合酶链式反应研究所鉴定的生物活性多肽对HTR-8/SVneo细胞功能的影响。共鉴定出3 92个上调多肽和4 2 0个下调多肽(绝对折叠变化 ≥ 2和调整的P值 <； 0.0 5)。GO分析、通路分析和IPA分析表明，这些差异表达的多肽在PE中起作用。此外，来自血管紧张素原的上调肽“DQSATALHFLGRVANPLSTA”对HTR-8/SVneo细胞的侵袭力也有一定的影响。目前的初步研究不仅为研究PE的发病机制提供了新的研究方向，也为PE的预测、诊断和治疗带来了新的见解。
This study sought to identify potential bioactive peptides from the placenta that are involved in preeclampsia (PE) to obtain information about the prediction, diagnosis and treatment of PE. The liquid chromatography/mass spectrometry was used to perform a comparative analysis of placental peptides in normal and PE pregnancies. Gene ontology (GO), pathway analysis and ingenuity pathway analysis (IPA) were used to evaluate the underlying biological function of the differential peptides based on their protein precursors. Transwell assays and qPCR were used to study the effect of the identified bioactive peptides on the function of HTR-8/SVneo cells. A total of 392 upregulated peptides and 420 downregulated peptides were identified (absolute fold change ≥ 2 and adjusted P value < 0.05). The GO analysis, pathway analysis, and IPA revealed that these differentially expressed peptides play a role in PE. In addition, the up-regulated peptide “DQSATALHFLGRVANPLSTA” derived from Angiotensinogen exhibited effect on the invasiveness of HTR-8/SVneo cells. The current preliminary research not only provides a new research direction for studying the pathogenesis of PE, but also brings new insights for the prediction, diagnosis and treatment of PE.
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在产后时期，从头和持续性高血压的流行病学和机制。
影响因子：
37.8
作者：
Goel A;Maski MR;Bajracharya S;Wenger JB;Zhang D;Salahuddin S;Shahul SS;Thadhani R;Seely EW;Karumanchi SA;Rana S
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Zhu Y
DOI：
10.1111/1471-0528.12375
发表时间：
2013-10-01
影响因子：
5.8
作者：
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影响因子：
11.8
作者：
Chng, Serene C.;Ho, Lena;Reversade, Bruno
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Reversade, Bruno
影响因子：
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作者：
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通讯作者：
Mumford SL