Block copolymer arrangement and composition effects on protein conformation using atomic force microscope-based antigen-antibody adhesion.
Block copolymer arrangement and composition effects on protein conformation using atomic force microscope-based antigen-antibody adhesion.
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DOI：
10.1002/jbm.a.34033
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发表时间：
2012-04
影响因子：
4.9
通讯作者：
Bhushan, B.
中科院分区：
文献类型：
--
作者：
Palacio, M. L. B.;Schricker, S. R.;Bhushan, B.
利用功能化的原子力显微镜（AFM）探针研究了纤维连接蛋白（FN）在聚甲基丙烯酸甲酯（PMMA）和聚丙烯酸（PAA）或聚甲基丙烯酸羟乙酯（PHEMA）嵌段共聚物上的构象变化。用对纤连蛋白中精氨酸-甘氨酸-天冬氨酸（RGD）基团的暴露敏感的抗体修饰尖端。通过研究抗体与吸附在嵌段共聚物表面的蛋白质之间的粘附相互作用和相位成像，发现具有大的微区尺寸的PAA-b-PMMA-b-PAA和PMMA-b-PHEMA-b-PMMA三嵌段共聚物有利于表面上的纤连蛋白RGD基团的暴露。基于这些结果，可以得出结论，表面化学以及纳米形态所规定的嵌段共聚物的安排可以调整蛋白质的构象和方向，并优化细胞粘附到生物材料表面。
The conformational changes of fibronectin deposited on various block copolymers where one block is composed of poly(methyl methacrylate) (PMMA) and the other block is either poly(acrylic acid) (PAA) or poly(2-hydroxyethyl methacrylate) (PHEMA) were investigated using a functionalized atomic force microscope (AFM) tip. The tip was modified with an antibody sensitive to the exposure of the arginine-glycine-aspartic acid (RGD) groups in fibronectin. By studying the adhesive interactions between the antibody and the proteins adsorbed on the block copolymer surface and phase imaging, it was found that the triblock copolymers PAA-b-PMMA-b-PAA and PMMA-b-PHEMA-b-PMMA, which both have large domain sizes, are conducive to the exposure of the fibronectin RGD groups on the surface. Based on these results, it is concluded that the surface chemistry as well as the nanomorphology dictated by the block copolymer arrangement could both tune protein conformation and orientation and optimize cell adhesion to the biomaterial surface.
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影响因子：
4.9
作者：
Kowalczynska, HM;Nowak-Wyrzykowska, M;Kaminski, J
通讯作者：
Kaminski, J
影响因子：
4.9
作者：
Baugh, L;Vogel, V
通讯作者：
Vogel, V
影响因子：
5.6
作者：
DICKINSON, CD;VEERAPANDIAN, B;ELY, KR
通讯作者：
ELY, KR
影响因子：
14
作者：
Lan, MA;Gersbach, CA;García, AJ
通讯作者：
García, AJ
DOI：
10.1002/jbm.10043
发表时间：
2002-06-05
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Collier, TO;Anderson, JM
通讯作者：
Anderson, JM