Neural crest stem cell-specific deletion of the Pygopus2 gene modulates hair follicle development.
Neural crest stem cell-specific deletion of the Pygopus2 gene modulates hair follicle development.
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DOI：
10.1007/s12015-013-9466-z
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发表时间：
2014-02
影响因子：
4.8
通讯作者：
Sieber-Blum, Maya
中科院分区：
文献类型：
--
作者：
Narytnyk, Alla;Gillinder, Kevin;Verdon, Bernard;Clewes, Oliver;Sieber-Blum, Maya
我们发现神经嵴干细胞影响小鼠毛囊发育。在胚胎发生过程中，毛囊的诱导受表皮和真皮层之间复杂的相互作用和功能冗余的信号调控，这一机制尚不完全清楚。典型的Wnt信号是神经嵴细胞的标志，也是表皮发位形成之前毛囊诱导的先决条件。由于神经嵴干细胞在胚胎早期发育过程中侵入表皮，我们旨在确定神经嵴细胞是否影响毛囊发育。为了减弱而不是沉默神经嵴细胞中的典型Wnt信号，我们分析了在神经嵴细胞中特异性缺失β-连环蛋白共激活因子基因Pygopus 2（Pygo2）的Wnt1-cre(+/−)::Pygo2（−/−）小鼠。突变小鼠的毛发密度和毛发厚度都减少了。此外，头发发育迟缓，头发类型的相对比例受到影响。锯齿状毛发减少，锥状毛发增加。小鼠神经嵴干细胞表达外胞质异常蛋白，这是形成锯齿状毛发的重要效应因子。综上所述，我们的数据支持神经嵴细胞参与毛囊发育早期阶段的新观点。
We show that neural crest stem cells affect mouse hair follicle development. During embryogenesis hair follicle induction is regulated by complex reciprocal and functionally redundant signals between epidermis and dermis, which remain to be fully understood. Canonical Wnt signalling is a hallmark of neural crest cells and also a prerequisite for hair follicle induction prior to hair placode formation in the epidermis. As neural crest stem cells invade the epidermis during early embryonic development we aimed at determining whether neural crest cells affect hair follicle development. To attenuate, but not silence, canonical Wnt signalling specifically in neural crest cells, we analyzed Wnt1-cre(+/−)::Pygo2(−/−) mice in which the β-catenin co-activator gene, Pygopus 2 (Pygo2), is deleted specifically in neural crest cells. Both, hair density and hair thickness were reduced in mutant mice. Furthermore, hair development was delayed and the relative ratio of hair types was affected. There was a decrease in zig-zag hairs and an increase in awl hairs. Mouse neural crest stem cells expressed ectodysplasin, an essential effector in the formation of zig-zag hair. Taken together, our data support the novel notion that neural crest cells are involved in the earliest stages of hair follicle development.
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影响因子：
4.8
作者：
Clewes, Oliver;Narytnyk, Alla;Gillinder, Kevin R.;Loughney, Andrew D.;Murdoch, Alison P.;Sieber-Blum, Maya
通讯作者：
Sieber-Blum, Maya
影响因子：
5.2
作者：
Hu, Yao Fei;Zhang, Zhi-Jian;Sieber-Blum, Maya
通讯作者：
Sieber-Blum, Maya
影响因子：
30.8
作者：
Headon, DJ;Overbeek, PA
通讯作者：
Overbeek, PA
影响因子：
2.6
作者：
Mikkola, ML;Pispa, J;Thesleff, I
通讯作者：
Thesleff, I
影响因子：
4.3
作者：
Gu, Bingnan;Watanabe, Kazuhide;Dai, Xing
通讯作者：
Dai, Xing