Human epidermal neural crest stem cells (hEPI-NCSC)--characterization and directed differentiation into osteocytes and melanocytes.
Human epidermal neural crest stem cells (hEPI-NCSC)--characterization and directed differentiation into osteocytes and melanocytes.
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DOI：
10.1007/s12015-011-9255-5
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发表时间：
2011-11
影响因子：
4.8
通讯作者：
Sieber-Blum, Maya
中科院分区：
文献类型：
--
作者：
Clewes, Oliver;Narytnyk, Alla;Gillinder, Kevin R.;Loughney, Andrew D.;Murdoch, Alison P.;Sieber-Blum, Maya
关键词：
在这里，我们描述了人表皮神经嵴干细胞（hEPI-NCSC）的分离、表征和离体扩增，并提供了它们定向分化为骨细胞和黑素细胞的方案。 hEPI-NCSC 是神经嵴衍生的多能干细胞，在毛囊隆起处持续存在至成年期。通过体外克隆分析确定多能性和自我更新。 hEPI-NCSC 产生所有主要的神经嵴衍生物，包括骨/软骨细胞、神经元、雪旺细胞、肌成纤维细胞和黑素细胞。此外，hEPI-NCSC 表达额外的神经嵴干细胞标记和全局干细胞基因。 hEPI-NCSC 在不同程度上以供体依赖性方式表达六种必需多能性基因 C-MYC、KLF4、SOX2、LIN28、OCT-4/POU5F1 和 NANOG。 hEPI-NCSC 可以离体扩增成数百万个干细胞，这些干细胞保持多能性并继续表达干细胞基因。 hEPI-NCSC 的新颖性在于其高度理想的特性的组合。 hEPI-NCSC 是出生后位置（毛囊凸起）的胚胎残余物。因此，通过微创手术可以很容易地在毛茸茸的皮肤中接触到它们。 hEPI-NCSC 是多能成体干细胞，可重复且高产量地分离。通过利用其迁移能力，hEPI-NCSC 可以作为高纯度的干细胞群体进行分离。 hEPI-NCSC 可以进行强大的离体扩增和定向分化。 hEPI-NCSC作为成体干细胞，有利于自体移植，避免移植排斥。这些特性共同使 hEPI-NCSC 成为未来细胞疗法和再生医学的新颖且有吸引力的候选者。本文的在线版本 (doi:10.1007/s12015-011-9255-5) 包含补充材料，可供授权用户使用。
Here we describe the isolation, characterisation and ex-vivo expansion of human epidermal neural crest stem cells (hEPI-NCSC) and we provide protocols for their directed differentiation into osteocytes and melanocytes. hEPI-NCSC are neural crest-derived multipotent stem cells that persist into adulthood in the bulge of hair follicles. Multipotency and self-renewal were determined by in vitro clonal analyses. hEPI-NCSC generate all major neural crest derivatives, including bone/cartilage cells, neurons, Schwann cells, myofibroblasts and melanocytes. Furthermore, hEPI-NCSC express additional neural crest stem cell markers and global stem cell genes. To variable degrees and in a donor-dependent manner, hEPI-NCSC express the six essential pluripotency genes C-MYC, KLF4, SOX2, LIN28, OCT-4/POU5F1 and NANOG. hEPI-NCSC can be expanded ex vivo into millions of stem cells that remain mulitpotent and continue to express stem cell genes. The novelty of hEPI-NCSC lies in the combination of their highly desirable traits. hEPI-NCSC are embryonic remnants in a postnatal location, the bulge of hair follicles. Therefore they are readily accessible in the hairy skin by minimal invasive procedure. hEPI-NCSC are multipotent somatic stem cells that can be isolated reproducibly and with high yield. By taking advantage of their migratory ability, hEPI-NCSC can be isolated as a highly pure population of stem cells. hEPI-NCSC can undergo robust ex vivo expansion and directed differentiation. As somatic stem cells, hEPI-NCSC are conducive to autologous transplantation, which avoids graft rejection. Together, these traits make hEPI-NCSC novel and attractive candidates for future cell-based therapies and regenerative medicine. The online version of this article (doi:10.1007/s12015-011-9255-5) contains supplementary material, which is available to authorized users.
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影响因子：
5.2
作者：
Hu, Yao Fei;Zhang, Zhi-Jian;Sieber-Blum, Maya
通讯作者：
Sieber-Blum, Maya
影响因子：
21.3
作者：
Fernandes, KJL;McKenzie, IA;Miller, FD
通讯作者：
Miller, FD
影响因子：
4.2
作者：
Hayashi, Ousuke;Katsube, Yoshihiro;Ito, Hiromoto
通讯作者：
Ito, Hiromoto
影响因子：
4.8
作者：
Hu, Yao Fei;Gourab, Krishnaj;Wells, Clive;Clewes, Oliver;Schmit, Brian D.;Sieber-Blum, Maya
通讯作者：
Sieber-Blum, Maya
影响因子：
2.7
作者：
LangtimmSedlak, CJ;Schroeder, B;SieberBlum, M
通讯作者：
SieberBlum, M