Advances in acrylamide bioproduction catalyzed with Rhodococcus cells harboring nitrile hydratase
Advances in acrylamide bioproduction catalyzed with Rhodococcus cells harboring nitrile hydratase
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含有腈水合酶的红球菌细胞催化丙烯酰胺生物生产的进展
DOI：
10.1007/s00253-019-10284-5
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发表时间：
2019-12
影响因子：
5
通讯作者：
Zhongyao Shen
中科院分区：
文献类型：
--
作者：
Song Jiao;Fulong Li;Huimin Yu;Zhongyao Shen
丙烯酰胺是生产聚丙烯酰胺的重要原料，广泛应用于三次采油、水处理等领域。用一种上级生物催化剂，含腈水合酶（NHase）的自由静息红球菌细胞生产丙烯酰胺，已被证明是简单而有效的，从而成为迄今为止工业上采用的主要方法。然而，在苛刻的工业条件下，天然状态的含NH酶的红球菌细胞易于失活。因此，已经报道了能够在酶或细胞水平进化重组红球菌生物催化剂的多种遗传策略。虽然大多数酶工程方法都集中在通过加强柔性位点来提高腈水合酶的稳定性，但采用各种方法进行红球菌细胞工程可以同时提高腈水合酶的活性和稳定性。开发新型反应器，特别是微型反应器，也是提高水化过程效率的有效途径。与传统搅拌釜反应器相比，膜分散微反应器可以增强以红球菌细胞为生物催化剂的水合过程中的传热和传质，从而显着提高丙烯酰胺生物生产过程的生产率。
Acrylamide is an important bulk chemical used for producing polyacrylamide, which is widely applied in diverse fields, such as enhanced oil recovery and water treatment. Acrylamide production with a superior biocatalyst, free-resting Rhodococcus cells containing nitrile hydratase (NHase), has been proven to be simple but effective, thereby becoming the main method adopted in industry to date. Under the harsh industrial conditions, however, NHase-containing Rhodococcus cells in a natural state are prone to deactivation. Thus, multiple genetic strategies able to evolve recombinant Rhodococcus biocatalysts at either the enzyme or cell level have been reported. While most of the methods on enzyme engineering concentrate on NHase stability enhancement by strengthening the flexible sites, Rhodococcus cell engineering with various methods can enhance both the NHase activity and stability as well. Developing some new types of reactors, especially the microreactor, is also an effective way to improve the hydration process efficiency. Compared with the conventional stirred tank reactor, the membrane dispersion microreactor can enhance the heat and mass transfer in the hydration process with Rhodococcus cells as biocatalysts, thereby significantly improving the productivity of the acrylamide bioproduction process.
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影响因子：
2.2
作者：
Takihara H;Ogihara J;Yoshida T;Okuda S;Nakajima M;Iwabuchi N;Sunairi M
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DOI：
10.1002/9780470054581.eib004
发表时间：
2010-04
期刊：
--
影响因子：
--
作者：
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通讯作者：
R. Zheng;Yuguo Zheng;Yinchu Shen
来自红球菌的新型分子伴侣 GroEL2 及其与腈水合酶的融合嵌合体，共同增强活性和稳定性
影响因子：
4.7
作者：
Yangzi Chen;Song Jiao;Miaomiao Wang;Jie Chen;Huimin Yu
通讯作者：
Huimin Yu
影响因子：
5
作者：
Song Jiao;Jie Chen;Huimin Yu;Zhongyao Shen
通讯作者：
Song Jiao;Jie Chen;Huimin Yu;Zhongyao Shen