The protein L-isoaspartyl-O-methyltransferase functions in the Caenorhabditis elegans stress response.
The protein L-isoaspartyl-O-methyltransferase functions in the Caenorhabditis elegans stress response.
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DOI：
10.1016/j.mad.2008.09.019
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发表时间：
2008-12
影响因子：
5.3
通讯作者：
Clarke, Steven G.
中科院分区：
文献类型：
--
作者：
Gomez, Tara A.;Banfield, Kelley L.;Clarke, Steven G.
关键词：
营养物质的有效利用对发育和衰老至关重要。在这项研究中，我们询问蛋白质修复甲基转移酶是否在秀丽隐杆线虫的能量代谢和应激反应中具有相关或额外的作用。缺乏编码该酶的pcm-1基因的线虫在sds分离的daer幼虫和饥饿胁迫下被捕获的L1幼虫中表现出寿命降低，而过表达导致成虫寿命增加。这些发现使我们质疑，缺乏pcm-1的秀丽隐杆线虫是否可能对应激有不适当的代谢反应。我们检测了dawer和dawer样幼虫的饥饿存活率，发现与N2野生型蠕虫相比，pcm-1突变体的中位存活时间减少了2倍。在这些条件下，缺乏pcm-1的达尔幼虫脂肪储存减少，这表明pcm-1可能在启动应激饥饿的正确代谢反应中起作用。我们发现pcm-1基因在神经元、体壁和生殖组织中表达。在热休克和水形成诱导条件下，我们在体壁肌核、肌动球蛋白丝和皮下细胞中观察到额外的pcm-1表达。这些结果表明，这种酶可能在应激反应途径中很重要，包括正确的能量储存决策。
The efficient use of nutrients is important in development and aging. In this study, we asked if the protein repair methyltransferase has a related or additional role in energy metabolism and stress response in the nematode Caenorhabditis elegans. Worms lacking the pcm-1 gene encoding this enzyme exhibit reduced longevity as SDS-isolated dauer larvae and as arrested L1 larvae under starvation stress, while overexpression leads to increased adult longevity. These findings led us to question whether pcm-1 deficient C. elegans may have inappropriate metabolic responses to stress. We assayed dauer and dauer-like larvae for starvation survival and observed a 2-fold reduction of median survival time for pcm-1 mutants compared to N2 wild-type worms. Under these conditions, pcm-1 deficient dauer larvae had reduced fat stores, suggesting that PCM-1 may have a role in the initiation of the correct metabolic responses to stress starvation. We show expression of the pcm-1 gene in neurons, body wall and reproductive tissues. Upon heat shock and dauer formation-inducing conditions, we observe additional pcm-1 expression in body wall muscle nuclei and actomyosin filaments and in hypodermal cells. These results suggest that this enzyme may be important in stress response pathways, including proper decision making for energy storage.
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DOI：
10.1093/gerona/63.8.798
发表时间：
2008-08-01
影响因子：
5.1
作者：
Banfield, Kelley L.;Gomez, Tara A.;Larsen, Pamela L.
通讯作者：
Larsen, Pamela L.
影响因子：
7.8
作者：
Farrar, C;Houser, CR;Clarke, S
通讯作者：
Clarke, S
影响因子：
64.8
作者：
Herndon, LA;Schmeissner, PJ;Driscoll, M
通讯作者：
Driscoll, M
影响因子：
3.7
作者：
Lanthier, J;Desrosiers, RR
通讯作者：
Desrosiers, RR
影响因子：
64.8
作者：
KLASS, M;HIRSH, D
通讯作者：
HIRSH, D