Immunosensor with fluid control mechanism for salivary cortisol analysis.
Immunosensor with fluid control mechanism for salivary cortisol analysis.
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DOI：
10.1016/j.bios.2012.08.016
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发表时间：
2013-03-15
影响因子：
12.6
通讯作者：
Shetty, Vivek
中科院分区：
文献类型：
--
作者：
Yamaguchi, Masaki;Matsuda, Yohei;Sasaki, Shohei;Sasaki, Makoto;Kadoma, Yoshihiro;Imai, Yoshikatsu;Niwa, Daisuke;Shetty, Vivek
The purpose of this research is to demonstrate a new design for a cortisol immunosensor for the noninvasive and quantitative analysis of salivary cortisol. We propose a cortisol immunosensor with a fluid control mechanism which has both a vertical flow and a lateral flow. The detected current resulting from a competitive reaction between the sample cortisol and a glucose oxidase (GOD)-labeled cortisol conjugate was found to be inversely related to the concentration of cortisol in the sample solution. A calibration curve using the relative detected current showed an R2 = 0.98 and CV = 14% for a range of standard cortisol solutions corresponding to the concentrations of native salivary cortisol (0.1 – 10 ng/ml). The measurement could be accomplished within 35 minutes and the cortisol immunosensor could be reused. These results show promise for realizing an on-site and easy-to-use biosensor for cortisol. Used for evaluation of human salivary cortisol levels, the cortisol immunosensor measurement corresponded closely with commercially available ELISA method (R2 = 0.92). Our results indicate the promise of the new cortisol immunosensor for noninvasive, point-of care measurement of human salivary cortisol levels.
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DOI：
10.1016/s0378-4347(99)00151-6
发表时间：
1999-06-25
期刊：
JOURNAL OF CHROMATOGRAPHY B
影响因子：
--
作者：
Rao, LV;Petersen, JR;Mohammad, AA
通讯作者：
Mohammad, AA
影响因子：
2
作者：
ATTILI, BS;SULEIMAN, AA
通讯作者：
SULEIMAN, AA
影响因子：
56.9
作者：
Lamberts, SWJ;vandenBeld, AW;vanderLely, AJ
通讯作者：
vanderLely, AJ
用流过滤的，便携式的表面等离子体共振生物传感器系统检测唾液中皮质醇的检测。
影响因子：
7.4
作者：
Stevens, Richard C.;Soelberg, Scott D.;Near, Steve;Furlong, Clement E.
通讯作者：
Furlong, Clement E.
DOI：
10.1016/s0378-4347(99)00550-2
发表时间：
2000-02-11
期刊：
JOURNAL OF CHROMATOGRAPHY B
影响因子：
--
作者：
Furuta, T;Matsuzawa, M;Kasuya, Y
通讯作者：
Kasuya, Y