Detection of cortisol in saliva with a flow-filtered, portable surface plasmon resonance biosensor system.
Detection of cortisol in saliva with a flow-filtered, portable surface plasmon resonance biosensor system.
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用流过滤的，便携式的表面等离子体共振生物传感器系统检测唾液中皮质醇的检测。
DOI：
10.1021/ac800892h
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发表时间：
2008-09-01
影响因子：
7.4
通讯作者：
Furlong, Clement E.
中科院分区：
文献类型：
--
作者：
Stevens, Richard C.;Soelberg, Scott D.;Near, Steve;Furlong, Clement E.
唾液为许多生物医学诊断测定提供了血液的有用和非侵入性替代品。血液和唾液中的皮质醇激素水平与压力水平有关。我们在这里提出了一个便携式表面等离子体共振（SPR）生物传感器系统检测唾液中的皮质醇的发展。皮质醇特异性单克隆抗体被用来开发一种竞争分析与6通道便携式SPR生物传感器在我们的实验室设计。实验室缓冲液中皮质醇的检测限为0.36 ng/ml（1.0 nM）。基于通过中空纤维亲水膜的扩散的在线过滤器用于在引入传感器表面之前从唾液的复杂大分子基质中分离小分子。过滤流动池提供了小分子与唾液粘蛋白和其他大分子的在线分离，与相同浓度的分析物在传感器表面上的直接流动相比，信号仅减少29%。生成了用于检测唾液中皮质醇的标准曲线，检测限为1.0 ng/ml（3.6 nM），对于临床使用足够灵敏。该系统也将是有用的小分子量的分析物在复杂的矩阵中发现的广泛的应用。
Saliva provides a useful and non-invasive alternative to blood for many biomedical diagnostic assays. The level of the hormone cortisol in blood and saliva is related to the level of stress. We present here the development of a portable surface plasmon resonance (SPR) biosensor system for detection of cortisol in saliva. Cortisol-specific monoclonal antibodies were used to develop a competition assay with a 6-channel portable SPR biosensor designed in our laboratory. The detection limit of cortisol in laboratory buffers was 0.36 ng/ml (1.0 nM). An in-line filter based on diffusion through a hollow fiber hydrophilic membrane served to separate small molecules from the complex macromolecular matrix of saliva prior to introduction to the sensor surface. The filtering flow cell provided in-line separation of small molecules from salivary mucins and other large molecules with only a 29% reduction of signal compared with direct flow of the same concentration of analyte over the sensor surface. A standard curve for detection of cortisol in saliva was generated with a detection limit of 1.0 ng/ml (3.6 nM), sufficiently sensitive for clinical use. The system will also be useful for a wide range of applications where small molecular weight analytes are found in complex matrices.
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影响因子：
5.8
作者：
Knutsson, U;Dahlgren, J;AlbertssonWikland, K
通讯作者：
AlbertssonWikland, K
DOI：
10.1016/s1570-0232(02)00753-5
发表时间：
2003-01-25
影响因子：
3
作者：
Jönsson, BAG;Malmberg, B;Orbæk, P
通讯作者：
Orbæk, P
影响因子：
4.3
作者：
Mauriz, E.;Calle, A.;Lechuga, L. M.
通讯作者：
Lechuga, L. M.
影响因子：
4.7
作者：
Adamczyk, M;Johnson, DD;Pan, Y
通讯作者：
Pan, Y
影响因子：
8.4
作者：
Chinowsky, TM;Quinn, JG;Elkind, JL
通讯作者：
Elkind, JL