The formulation of aptamer-coated paclitaxel-polylactide nanoconjugates and their targeting to cancer cells.
The formulation of aptamer-coated paclitaxel-polylactide nanoconjugates and their targeting to cancer cells.
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DOI：
10.1016/j.biomaterials.2010.01.009
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发表时间：
2010-04
期刊：
影响因子：
14
通讯作者：
Cheng, Jianjun
中科院分区：
文献类型：
--
作者：
Tong, Rong;Yala, Linda;Fan, Timothy M.;Cheng, Jianjun
通过紫杉醇/(BDI)锌(TMS)2(BDI=丙交酯(LA)的2-((2，6-diisopropylphenyl)-amido)-4-((2，6-diisopropylphenyl)-imino)-2-pentene)-mediated可控聚合-纳米沉淀法)制备了紫杉醇-聚乳酸(PTXL-PLA)纳米复合纳米粒。Ptxl-聚乳酸的纳米沉淀得到了粒径小于100 nm的纳米颗粒，颗粒呈单峰分布，多分散性较低。在纳米沉淀过程中，通过选择合适的水相容溶剂和控制Ptxl-聚乳酸的浓度，可以精确控制Ptxl-PLANCS的大小。聚乳酸-聚乙二醇-聚乳酸(聚乳酸=14 kDa；聚乙二醇=5 kDa)与Ptx1-聚乳酸在PBS中的共沉淀可使NCS在PBS中保持较长时间的非聚集状态。为了开发NCS的固体制剂，我们对一系列冷冻保护剂进行了评估，旨在确定在冷冻干燥过程中可以有效减少或消除NC聚集的候选物质。白蛋白被发现是一种很好的固体形式的NCS的冻存保护剂，允许冻干的NCS很容易分散在PBS中，而没有明显的聚集。制备了适体-NCS生物偶联物，发现其能够以细胞特异性的方式有效地靶向前列腺特异性膜抗原。
Paclitaxel-polylactide (Ptxl-PLA) conjugate nanoparticles, termed as nanoconjugates (NCs), were prepared through Ptxl/(BDI)ZnN(TMS)2 (BDI = 2-((2,6-diisopropylphenyl)-amido)-4-((2,6-diisopropylphenyl)-imino)-2-pentene)-mediated controlled polymerization of lactide (LA) followed by nanoprecipitation. Nanoprecipitation of Ptxl-PLA resulted in sub-100 nm NCs with monomodal particle distributions and low polydispersities. The sizes of Ptxl-PLA NCs could be precisely controlled by using appropriate water-miscible solvents and by controlling the concentration of Ptxl-PLA during nanoprecipitation. Co-precipitation of a mixture of PLA-PEG-PLA (PLA = 14 kDa; PEG = 5kDa) and Ptxl-PLA in PBS resulted in NCs that could stay non-aggregated in PBS for an extended period of time. To develop solid formulations of NCs, we evaluated a series of lyoprotectants, aiming to identify candidates that could effectively reduce or eliminate NC aggregation during lyophilization. Albumin was found to be an excellent lyoprotectant for the preparation of NCs in solid form, allowing lyophilized NCs to be readily dispersed in PBS without noticeable aggregates. Aptamer-NCs bioconjugates were prepared and found to be able to effectively target prostate-specific membrane antigen in a cell-specific manner.
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影响因子：
4.9
作者：
Alexis F;Pridgen E;Molnar LK;Farokhzad OC
通讯作者：
Farokhzad OC
影响因子：
5.8
作者：
Musumeci, T.;Ventura, C. A.;Puglisi, G.
通讯作者：
Puglisi, G.
影响因子：
3.7
作者：
Galindo-Rodriguez, S;Allémann, E;Doelker, E
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Doelker, E
影响因子：
64.8
作者：
ELLINGTON, AD;SZOSTAK, JW
通讯作者：
SZOSTAK, JW
影响因子：
14
作者：
Cheng, Jianjun;Teply, Benjamin A.;Farokhzad, Omid C.
通讯作者：
Farokhzad, Omid C.