Comparative proteomic analysis identifies proteins associated with the development and progression of colorectal carcinoma
Comparative proteomic analysis identifies proteins associated with the development and progression of colorectal carcinoma
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比较蛋白质组学分析鉴定与结直肠癌发生和进展相关的蛋白质
DOI：
10.1111/j.1742-4658.2010.07808.x
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发表时间：
2010-10
期刊：
影响因子：
5.4
通讯作者：
Ding, Yanqing
中科院分区：
文献类型：
--
作者：
Zhao, Liang;Wang, Hui;Sun, Xuegang;Ding, Yanqing
为了更好地了解结直肠癌（CRC）发生或转移的机制，并寻找CRC预后的潜在标志物，对CRC组织进行了比较蛋白质组学分析。从正常结直肠粘膜、非转移性CRC（nmCRC）和转移性CRC（mCRC）组织样本中提取蛋白质。通过二维电泳结合MALDI-TOF MS对每份样本进行蛋白质分析，然后通过蛋白质印迹法进行确认。发现31种蛋白质在正常粘膜、nmCRC和mCRC组织之间差异表达。在126例石蜡包埋的结直肠癌标本中，通过免疫组化染色检测3种差异表达蛋白，经蛋白质组学分析鉴定为LASP-1、S100 A9和RhoGDI，以确定这些蛋白在结直肠癌中的临床病理特征。与正常粘膜相比，这些蛋白质在CRC中的表达水平增加，尤其是mCRC。这些结果为寻找大肠癌发生和转移的潜在标志物提供了基础，也为阐明大肠癌的进展机制提供了线索。所确定的模式变化有可能用于设计标记物组，以帮助CRC的诊断和治疗策略。
To better understand the mechanism underlying colorectal carcinoma (CRC) genesis or metastasis, and to search for potential markers for CRC prognosis, a comparative proteomic analysis was performed on CRC tissue. Proteins were extracted from normal colorectal mucosa, non‐metastatic CRC (nmCRC) and metastatic CRC (mCRC) tissue samples. Protein profiling of each sample was performed by two‐dimensional electrophoresis coupled with MALDI‐TOF MS, followed by confirmation by Western blotting. Thirty‐one proteins were found to be differentially expressed between normal mucosa, nmCRC and mCRC tissue. In 126 paraffin‐embedded CRC samples, three differentially expressed proteins, identified as LASP‐1, S100A9 and RhoGDI by proteomic analysis, were detected by immunohistochemical staining to determine the clinicopathological characteristics of these proteins in CRC. Increased expression levels of these proteins were found in CRC, especially mCRC, compared with normal mucosa. The results provide the basis for searching for potential markers for CRC genesis and metastasis, and also provide clues for elucidating the mechanism of CRC progression. The pattern changes identified have the potential to be used for the design of marker panels for assistance in diagnostic and therapeutic strategies in CRC.
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LASP-1 的过度表达介导人卵巢癌细胞的迁移和增殖并影响 zyxin 定位。
影响因子：
8.8
作者：
通讯作者：
--
DOI：
--
发表时间：
2001-05
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
S. Srivastava;M. Verma;Donald E. Henson
通讯作者：
S. Srivastava;M. Verma;Donald E. Henson
DOI：
10.1097/00042737-200109000-00006
发表时间：
2001-09
影响因子：
2.1
作者：
H. Wallace;R. Caslake
通讯作者：
H. Wallace;R. Caslake
影响因子：
4.8
作者：
Arozarena, I;Matallanas, D;Crespo, P
通讯作者：
Crespo, P
影响因子：
5.7
作者：
Tian, Tian;Hao, Jia;He, Dacheng
通讯作者：
He, Dacheng