Identification of Avramr1 from Phytophthora infestans using long read and cDNA pathogen-enrichment sequencing (PenSeq).
Identification of Avramr1 from Phytophthora infestans using long read and cDNA pathogen-enrichment sequencing (PenSeq).
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DOI：
10.1111/mpp.12987
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发表时间：
2020-11
影响因子：
4.9
通讯作者：
Jones JDG
中科院分区：
文献类型：
--
作者：
Lin X;Song T;Fairhead S;Witek K;Jouet A;Jupe F;Witek AI;Karki HS;Vleeshouwers VGAA;Hein I;Jones JDG
马铃薯晚疫病由卵菌病原体致病疫霉引起，严重阻碍马铃薯生产。最近，从野生茄属植物美洲茄中克隆了一种新的抗致病疫霉 (Rpi) 基因 Rpi-amr1。从致病疫霉中鉴定相应的公认效应子（无毒力或 Avr）基因是阐明其自然发生的序列变异的关键，这反过来又揭示了同源晚疫病抗性的潜在持久性。为了识别 Rpi-amr1 识别的致病疫霉效应子，我们筛选了可用的 RXLR 效应子文库，并对四种致病疫霉分离株使用长读长和 cDNA 病原体富集测序 (PenSeq)，以探索未经测试的效应子。使用单分子实时测序 (SMRT) 和 cDNA PenSeq，我们鉴定了致病疫霉中的 47 个高表达效应子，其中包括 PITG_07569，当与本塞姆氏烟草中的 Rpi-amr1 瞬时共表达时，它会触发高度特异性的细胞死亡反应，表明 PITG_07569 是 Avramr1。在这里，我们证明长读长和 cDNA PenSeq 能够识别全长 RXLR 效应子家族及其表达谱。这项研究揭示了与 Rpi-amr1 识别相关的复杂 RXLR 效应家族的进化和多态性的重要见解。开发了长读长和 cDNA 病原体富集测序 (PenSeq)，以揭示四种致病疫霉分离株的 RXLR 效应子库和表达模式，并能够鉴定新的无毒基因 Avramr1。
Potato late blight, caused by the oomycete pathogen Phytophthora infestans, significantly hampers potato production. Recently, a new Resistance to Phytophthora infestans (Rpi) gene, Rpi‐amr1, was cloned from a wild Solanum species, Solanum americanum. Identification of the corresponding recognized effector (Avirulence or Avr) genes from P. infestans is key to elucidating their naturally occurring sequence variation, which in turn informs the potential durability of the cognate late blight resistance. To identify the P. infestans effector recognized by Rpi‐amr1, we screened available RXLR effector libraries and used long read and cDNA pathogen‐enrichment sequencing (PenSeq) on four P. infestans isolates to explore the untested effectors. Using single‐molecule real‐time sequencing (SMRT) and cDNA PenSeq, we identified 47 highly expressed effectors from P. infestans, including PITG_07569, which triggers a highly specific cell death response when transiently coexpressed with Rpi‐amr1 in Nicotiana benthamiana, suggesting that PITG_07569 is Avramr1. Here we demonstrate that long read and cDNA PenSeq enables the identification of full‐length RXLR effector families and their expression profile. This study has revealed key insights into the evolution and polymorphism of a complex RXLR effector family that is associated with the recognition by Rpi‐amr1. Long reads and cDNA pathogen enrichment sequencing (PenSeq) are developed to reveal the RXLR effector repertoires and expression pattern of four Phytophthora infestans isolates and enable to identify a new avirulence gene Avramr1.
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DOI：
10.1111/tpj.12307
发表时间：
2013-11
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Jupe F;Witek K;Verweij W;Sliwka J;Pritchard L;Etherington GJ;Maclean D;Cock PJ;Leggett RM;Bryan GJ;Cardle L;Hein I;Jones JD
通讯作者：
Jones JD
影响因子：
30.3
作者：
Guo, Hailong;Ahn, Hee-Kyung;Jones, Jonathan D. G.
通讯作者：
Jones, Jonathan D. G.
影响因子：
7.2
作者：
Huang, SW;van der Vossen, EAG;Visser, RGF
通讯作者：
Visser, RGF
影响因子：
7.2
作者：
Ballvora, A;Ercolano, MR;Gebhardt, C
通讯作者：
Gebhardt, C
影响因子：
3.5
作者：
Oliva, Ricardo F.;Cano, Liliana M.;Kamoun, Sophien
通讯作者：
Kamoun, Sophien