Ultrastructural and biochemical analysis of the stress granule in chicken embryo fibroblasts.
Ultrastructural and biochemical analysis of the stress granule in chicken embryo fibroblasts.
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DOI：
10.1083/jcb.106.4.1131
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发表时间：
1988-04
期刊：
影响因子：
--
通讯作者：
Schlesinger MJ
中科院分区：
文献类型：
--
作者：
Collier NC;Heuser J;Levy MA;Schlesinger MJ
对鸡胚成纤维细胞在应激过程中形成的细胞质颗粒的超微结构和生化组成进行了分析。我们以前表明，这些颗粒含有小的应激蛋白，sp 24，和sp 24特异性抗体在这里被用来识别应激颗粒。在薄切片中，应力颗粒是一个染色密集，无膜，细胞质体，并出现在冷冻断裂制备的细胞质高度浓缩的区域。冷冻断裂分析前的细胞低渗肿胀揭示了互连的蛋白质电缆组成的篮状结构。当用二硫代双-（琥珀酰亚胺基丙酸酯）处理胁迫颗粒时，没有其他蛋白质可以与sp 24交联。高分辨率放射自显影分析与[3 H]尿苷未能确定任何相关的RNA合成的期间之前的压力。因此，应力颗粒似乎主要由sp 24聚集体组成。Sp24可以通过在8 M尿素中溶解和阴离子交换层析从胁迫颗粒中纯化至均一。
The ultrastructure and biochemical composition of cytoplasmic particles that form in chicken embryo fibroblasts during stress have been analyzed. We showed previously that these particles contained the small stress protein, sp 24, and antibodies specific to sp 24 were used here to identify the stress granule. In thin sections, the stress granule was a densely staining, membraneless, cytoplasmic body and appeared as a highly condensed area of cytoplasm in freeze-fracture preparations. Hypotonic swelling of cells before freeze-fracture analysis revealed a basketlike structure composed of interconnecting protein cables. No other proteins could be cross-linked to sp 24 when stress granules were treated with dithiobis-(succinimidyl propionate). High resolution autoradiographic analysis with [3H]uridine failed to identify any associated RNA synthesized in the period immediately before the stress. Thus the stress granule appears to be composed predominantly of sp 24 aggregates. Sp 24 could be purified to homogeneity from the stress granule by solubilization in 8 M urea and anion exchange chromatography.
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DOI：
10.1083/jcb.84.3.560
发表时间：
1980-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Heuser J
通讯作者：
Heuser J
影响因子：
5.6
作者：
HEUSER, JE
通讯作者：
HEUSER, JE
DOI：
10.1073/pnas.83.20.7638
发表时间：
1986-10-01
影响因子：
11.1
作者：
DODSON, M;ECHOLS, H;MCMACKEN, R
通讯作者：
MCMACKEN, R
影响因子：
5.3
作者：
KELLEY, PM;SCHLESINGER, MJ
通讯作者：
SCHLESINGER, MJ
影响因子：
2.7
作者：
ARRIGO, AP;FAKAN, S;TISSIERES, A
通讯作者：
TISSIERES, A