Plasmonic nanoparticles assemblies templated by helical bacteria and resulting optical activity.
Plasmonic nanoparticles assemblies templated by helical bacteria and resulting optical activity.
复制标题
作者：
Feng W;Kadiyala U;Yan J;Wang Y;DiRita VJ;VanEpps JS;Kotov NA
等离子体纳米粒子（NP）吸附到螺旋细菌上可以导致形成具有微米级螺距的NP螺旋体。相关的手旋光效应可以作为生物分析工具用于细菌检测和更好地理解不同尺度的螺旋自组装结构的光谱行为。在这里，我们报告说，对映体纯螺旋与微米尺度的手性可以组装空肠弯曲杆菌，螺旋细菌已知的严重的胃感染。这些生物体具有1-2微米螺距的右旋螺旋形状，并且可以用作各种NP的通用模板。细菌本身在电磁波谱的可见光、红光和近红外范围内没有可观察到的旋光活性。细菌分散体在用Au NP等离子体功能化后在500-750 nm处获得手性光活性。时间域的三阶差分模拟证实了手光学活性的属性的螺旋组装的金纳米粒子。对于这些手性结构观察到的圆二色谱峰的位置与之前对于具有分子和纳米级手性的Au NP及其构建体获得的那些重叠。这项工作提供了一个实验和计算的途径，利用手性粒子组装在细菌的生物分析目的。
Plasmonic nanoparticles (NPs) adsorbing onto helical bacteria can lead to formation of NP helicoids with micron scale pitch. Associated chiroptical effects can be utilized as bioanalytical tool for bacterial detection and better understanding of the spectral behavior of helical self-assembled structures with different scales. Here, we report that enantiomerically pure helices with micron scale of chirality can be assembled on Campylobacter jejuni, a helical bacterium known for severe stomach infections. These organisms have right-handed helical shapes with a pitch of 1–2 microns and can serve as versatile templates for a variety of NPs. The bacteria itself shows no observable rotatory activity in the visible, red, and near-IR ranges of electromagnetic spectrum. The bacterial dispersion acquires chiroptical activity at 500–750 nm upon plasmonic functionalization with Au NPs. Finite-difference time-domain simulations confirmed the attribution of the chiroptical activity to the helical assembly of gold nanoparticles. The position of the circular dichroism peaks observed for these chiral structures overlaps with those obtained before for Au NPs and their constructs with molecular and nanoscale chirality. This work provides an experimental and computational pathway to utilize chiroplasmonic particles assembled on bacteria for bioanalytical purposes.
登录
查看更多内容
影响因子：
6.4
作者：
French, Shawn;Cote, Jean-Philippe;Brown, Eric D.
通讯作者：
Brown, Eric D.
影响因子：
4.5
作者：
Balch, WM;Vaughn, JM;Ashe, A
通讯作者：
Ashe, A
DOI：
10.3390/ijerph10126292
发表时间：
2013-11-26
影响因子：
--
作者：
Epps SV;Harvey RB;Hume ME;Phillips TD;Anderson RC;Nisbet DJ
通讯作者：
Nisbet DJ
光学活性纳米结构 ZnO 薄膜
影响因子：
16.6
作者：
Duan, Yingying;Han, Lu;Che, Shunai
通讯作者：
Che, Shunai
影响因子：
56.9
作者：
Gansel, Justyna K.;Thiel, Michael;Wegener, Martin
通讯作者：
Wegener, Martin