Differences in the antibody response to adult Fasciola hepatica excretory/secretory products in experimentally and naturally infected cattle and sheep.
Differences in the antibody response to adult Fasciola hepatica excretory/secretory products in experimentally and naturally infected cattle and sheep.
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DOI：
10.1016/j.vetpar.2020.109321
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发表时间：
2021-01
影响因子：
2.6
通讯作者：
Williams D
中科院分区：
文献类型：
--
作者：
Walsh TR;Ainsworth S;Armstrong S;Hodgkinson J;Williams D
实验性和自然感染肝镰刀菌的动物的抗体反应是不同的。实验感染的动物特异性识别组织蛋白酶蛋白。自然感染的动物对重组组织蛋白酶 L1 的识别能力较差。自然感染动物的抗体反应针对多种抗原。基于单一抗原的诊断测试可能不适合在现场使用。肝片形吸虫（肝吸虫）是一种常见的全球性牲畜寄生虫。它可能具有高致病性，并对感染者的健康和福利产生影响。通常，在反刍动物中，感染是亚临床的，但如果未确诊，可能会导致重大的生产损失。准确诊断对于识别感染至关重要。抗体检测 ELISA 由于其高灵敏度和特异性而常用于诊断感染，并且通常基于天然吸虫排泄/分泌 (ES) 产品或组织蛋白酶 L1 (CL1)（ES 产品中的免疫显性抗原）。这些测试是根据实验感染动物的抗体反应而开发的；然而，这种反应在自然感染的动物中尚未得到很好的表征。我们比较了重组 CL1 (rCL1) 抗原和天然成人吸虫 ES 产品的抗体识别能力。虽然来自实验感染动物的样本显示出对 rCL1 的强烈识别，但来自自然感染动物的血清抗体却没有。这些结果通过肽阵列得到证实。针对 ES 产品的免疫印迹血清显示，实验感染的动物对 CL1/CL2 蛋白具有强烈的特异性反应，而来自自然感染动物的抗体可识别多种蛋白，并对 CL1/CL2 具有不同的反应。通过 2D SDS PAGE 分离的蛋白质进行质谱分析，鉴定出可被自然感染牛的血清抗体识别的几种抗原，包括组织蛋白酶 L1、L2 和 L5、谷胱甘肽 S-转移酶和二氢硫辛酰脱氢酶。总体而言，这些结果表明，自然感染动物对成年吸虫 ES 产品的抗体反应与实验感染动物有本质上的不同。这表明仅基于 CL1 的诊断测试可能不适合诊断绵羊和牛的天然肝镰刀菌感染。
Antibody response is different in animals experimentally and naturally infected with F. hepatica. Experimentally infected animals specifically recognised cathepsin proteins. Naturally infected animals showed poor recognition of a recombinant cathepsin L1. Antibody response of naturally infected animals is against multiple antigens. Diagnostic tests based on a single antigen may not be suitable for use in field. Fasciola hepatica (the liver fluke) is a common, global parasite of livestock. It can be highly pathogenic and has health and welfare implications for infected individuals. Typically, in ruminants, infections are sub-clinical, but if undiagnosed, they can lead to significant production losses. Accurate diagnosis is crucial to identify infection. Antibody detection ELISAs are commonly used to diagnose infection due to their high sensitivity and specificity and are typically based on native fluke excretory/secretory (ES) products or cathepsin L1 (CL1), the immunodominant antigen within ES products. These tests have been developed based on the antibody response of experimentally infected animals; however, this response has not been well characterised in naturally infected animals. We compared the antibody recognition of a recombinant CL1 (rCL1) antigen and native adult fluke ES products. Whilst samples from experimentally infected animals showed strong recognition of rCL1, serum antibodies from naturally infected animals did not. These results were confirmed by peptide array. Immunoblotting sera against ES products showed that experimentally infected animals had a strong, specific response to CL1/CL2 proteins whilst antibodies from naturally infected animals recognised multiple proteins and had a variable response to CL1/CL2. Mass spectrometry of proteins separated by 2D SDS PAGE, identified several antigens recognised by serum antibodies from a naturally infected cow, including cathepsins L1, L2 and L5, glutathione S-transferase and a dihydrolipoyl dehydrogenase. Overall, these results show that the antibody response in naturally infected animals to adult fluke ES products is qualitatively different to experimentally infected animals. This suggests that a diagnostic test based on CL1 alone may not be appropriate for diagnosis of natural F. hepatica infections in sheep and cattle.
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影响因子：
3.7
作者：
Charlier J;Hostens M;Jacobs J;Van Ranst B;Duchateau L;Vercruysse J
通讯作者：
Vercruysse J
影响因子：
4
作者：
CHAUVIN, A;BOUVET, G;BOULARD, C
通讯作者：
BOULARD, C
影响因子：
1.3
作者：
Berasain, P;Goni, F;Carmona, C
通讯作者：
Carmona, C
DOI：
10.2165/00063030-200721030-00002
发表时间：
2007
期刊：
BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy
影响因子：
--
作者：
Gershoni JM;Roitburd-Berman A;Siman-Tov DD;Tarnovitski Freund N;Weiss Y
通讯作者：
Weiss Y
影响因子：
3.2
作者：
Buffoni, Leandro;Garza-Cuartero, Laura;Mulcahy, Grace
通讯作者：
Mulcahy, Grace