Use of a rapid cytotoxicity screening approach to engineer a safer zinc oxide nanoparticle through iron doping.
Use of a rapid cytotoxicity screening approach to engineer a safer zinc oxide nanoparticle through iron doping.
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DOI：
10.1021/nn901503q
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发表时间：
2010-01-26
期刊：
影响因子：
17.1
通讯作者：
Nel, Andre E.
中科院分区：
文献类型：
--
作者：
George, Saji;Pokhrel, Suman;Xia, Tian;Gilbert, Benjamin;Ji, Zhaoxia;Schowalter, Marco;Rosenauer, Andreas;Damoiseaux, Robert;Bradley, Kenneth A.;Maedler, Lutz;Nel, Andre E.
建立可核查的安全纳米技术需要开发评估工具，以确定可以修改以提高纳米材料安全性的危险纳米材料特性。虽然关于什么是适当的安全筛选方法存在很多争论，但一种方法是使用细胞损伤途径的评估来收集有关可能导致人类和环境危害的危险材料特性的知识。我们证明了使用多参数细胞毒性试验，评估毒性氧化应激，比较二氧化钛（TiO 2），氧化铈（CeO 2）和氧化锌（ZnO）纳米粒子在支气管上皮细胞和巨噬细胞系的影响。纳米颗粒的选择是基于它们的产量和扩散到环境中的可能性。在这些材料中，ZnO纳米颗粒的溶解和Zn2+的释放能够产生ROS并激活包括细胞内钙通量、线粒体去极化和质膜渗漏的综合细胞毒性途径。这些响应是基于荧光染料的相容性选择的，所述荧光染料通过半自动落射荧光程序同时评估其响应特性。通过铁掺杂，改变材料基质以减缓Zn2+释放，实现了有目的地降低ZnO的细胞毒性。总之，我们证明了一个快速的吞吐量，集成的生物氧化应激反应途径的实用性，以执行一小批金属氧化物纳米颗粒的危害排名，除了显示如何使用这种测定可以通过减少ZnO溶解通过Fe掺杂来提高纳米安全性。
The establishment of verifiably safe nanotechnology requires the development of assessment tools to identify hazardous nanomaterial properties that could be modified to improve nanomaterial safety. While there is a lot of debate of what constitutes appropriate safety screening methods, one approach is to use the assessment of cellular injury pathways to collect knowledge about hazardous material properties that could lead to harm to humans and the environment. We demonstrate the use of a multi-parameter cytotoxicity assay that evaluates toxic oxidative stress to compare the effects of titanium dioxide (TiO2), cerium oxide (CeO2) and zinc oxide (ZnO) nanoparticles in bronchial epithelial and macrophage cell lines. The nanoparticles were chosen based on their volume of production and likelihood of spread to the environment. Among the materials, dissolution of ZnO nanoparticles and Zn2+ release were capable of ROS generation and activation of an integrated cytotoxic pathway that includes intracellular calcium flux, mitochondrial depolarization, and plasma membrane leakage. These responses were chosen based on the compatibility of the fluorescent dyes that contemporaneously assess their response characteristics by a semi-automated epifluorescence procedure. Purposeful reduction of ZnO cytotoxicity was achieved by iron doping, which changed the material matrix to slow Zn2+ release. In summary, we demonstrate the utility of a rapid throughput, integrated biological oxidative stress response pathway to perform hazard ranking of a small batch of metal oxide nanoparticles, in addition to showing how this assay can be used to improve nanosafety by decreasing ZnO dissolution through Fe doping.
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影响因子：
10.4
作者：
Li N;Wang M;Bramble LA;Schmitz DA;Schauer JJ;Sioutas C;Harkema JR;Nel AE
通讯作者：
Nel AE
影响因子：
10
作者：
Araujo JA;Nel AE
通讯作者：
Nel AE
影响因子：
56.9
作者：
Feng, Xiangdong;Sayle, Dean C.;Her, Yie-Shein
通讯作者：
Her, Yie-Shein
影响因子：
4.5
作者：
Mayer, Andrea;Vadon, Maria;Froehlich, Eleonore
通讯作者：
Froehlich, Eleonore
影响因子：
4.4
作者：
Li, N;Alam, J;Nel, AE
通讯作者：
Nel, AE