A novel small diameter nanotextile arterial graft is associated with surgical feasibility and safety and increased transmural endothelial ingrowth in pig.
A novel small diameter nanotextile arterial graft is associated with surgical feasibility and safety and increased transmural endothelial ingrowth in pig.
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DOI：
10.1186/s12951-022-01268-1
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发表时间：
2022-02-08
影响因子：
10.2
通讯作者：
Ascione R
中科院分区：
文献类型：
--
作者：
Joseph J;Domenico Bruno V;Sulaiman N;Ward A;Johnson TW;Baby HM;Kerala Varma P;Jose R;Nair SV;Menon D;George SJ;Ascione R
在全球范围内，数以百万计的患者因动脉粥样硬化而受到心肌梗死或下肢坏疽/截肢的影响。现有的基于静脉和合成移植物的外科治疗提供了不太理想的益处。我们设计了一种高度灵活和机械坚固的纳米织物血管移植物(NanoGraft)，通过交织聚L-乳酸的纳米纤维线来满足未得到满足的需求。通过预凝血，纳米移植物通过在微孔中选择性地沉积纤维蛋白而变得不透水。将预凝的纳米移植物(直径4 mm)和ePTFE植入猪颈动脉置换模型。富含纤维蛋白的多孔环境通过跨壁血管生成促进了纳米移植物的快速内皮化。纳米移植物的体内通畅率在2周和4周是100%，管腔大小、血流速度和最小的异物炎症反应没有随时间变化。然而，ePTFE在2周时的通畅率为66%，并显示出明显的浸润性、新生内膜增厚和宿主组织整合不良。这项研究证明了一种薄层血管假体，即NanoGraft的体内可行性和安全性，以及它相对于商用ePTFE的潜在优势。网上版载有补充材料，可在10.1186/s12951-022-01268-1查阅。
Globally, millions of patients are affected by myocardial infarction or lower limb gangrene/amputation due to atherosclerosis. Available surgical treatment based on vein and synthetic grafts provides sub-optimal benefits. We engineered a highly flexible and mechanically robust nanotextile-based vascular graft (NanoGraft) by interweaving nanofibrous threads of poly-L-lactic acid to address the unmet need. The NanoGrafts were rendered impervious with selective fibrin deposition in the micropores by pre-clotting. The pre-clotted NanoGrafts (4 mm diameter) and ePTFE were implanted in a porcine carotid artery replacement model. The fibrin-laden porous milieu facilitated rapid endothelization by the transmural angiogenesis in the NanoGraft. In-vivo patency of NanoGrafts was 100% at 2- and 4-weeks, with no changes over time in lumen size, flow velocities, and minimal foreign-body inflammatory reaction. However, the patency of ePTFE at 2-week was 66% and showed marked infiltration, neointimal thickening, and poor host tissue integration. The study demonstrates the in-vivo feasibility and safety of a thin-layered vascular prosthesis, viz., NanoGraft, and its potential superiority over the commercial ePTFE. The online version contains supplementary material available at 10.1186/s12951-022-01268-1.
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影响因子：
14
作者：
Ma, ZW;Kotaki, M;Ramakrishna, S
通讯作者：
Ramakrishna, S
影响因子：
4.3
作者：
Gibson, KD;Gillen, DL;Stehman-Breen, CO
通讯作者：
Stehman-Breen, CO
影响因子：
--
作者：
Berglund, JD;Nerem, RM;Sambanis, A
通讯作者：
Sambanis, A
影响因子：
4.3
作者：
Mrowczynski, Wojciech;Mugnai, Damiano;Walpoth, Beat H.
通讯作者：
Walpoth, Beat H.
影响因子：
14
作者：
Boccafoschi, F;Habermehl, J;Mantovani, D
通讯作者：
Mantovani, D