The antibacterial effect of photodynamic therapy in dental plaque-derived biofilms.
The antibacterial effect of photodynamic therapy in dental plaque-derived biofilms.
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DOI：
10.1111/j.1600-0765.2008.01187.x
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发表时间：
2009-12
影响因子：
3.5
通讯作者：
Soukos NS
中科院分区：
文献类型：
--
作者：
Fontana CR;Abernethy AD;Som S;Ruggiero K;Doucette S;Marcantonio RC;Boussios CI;Kent R;Goodson JM;Tanner AC;Soukos NS
光动力疗法（PDT）已被提倡作为一种替代抗菌药物，以抑制牙龈下的物种和治疗牙周炎。位于致密生物膜内的细菌，例如在牙菌斑中遇到的细菌，已被发现对抗菌治疗具有相对耐药性。在本研究中，我们通过比较亚甲基蓝（MB）对浮游期和生物膜中人牙菌斑微生物的光动力学作用，研究了PDT对生物膜中细菌耐药的影响。从10例慢性牙周炎患者身上采集牙菌斑样本。将5名受试者的菌斑微生物悬浮液用MB （25 μg/ml）致敏5分钟，然后暴露于红光下。从相同菌斑样品中形成的多物种微生物生物膜也暴露于MB （25 μg/ml）和与其浮游对应物相同的光照条件下。在第二组实验中，用5名受试者的菌斑细菌制备生物膜，分别用25和50 μg/ml MB致敏，然后照见光。PDT后，根据菌落形成单位计数计算生存分数。在悬浮液中，PDT的细菌杀灭率约为63%。在生物膜中，PDT的效果导致微生物的减少要低得多（最大杀死量的32%）。生物膜中的口腔细菌受PDT的影响要小于浮游期的细菌。PDT对生物膜细菌的抑菌效果有所降低，但与已有报道的在类似条件下用抗生素治疗的效果不同。
Photodynamic therapy (PDT) has been advocated as an alternative to antimicrobial agents to suppress subgingival species and treat periodontitis. Bacteria located within dense biofilms, such as those encountered in dental plaques, have been found to be relatively resistant to antimicrobial therapy. In the present study, we investigated the ability of PDT to affect bacteria resistant in biofilms by comparing the photodynamic effects of methylene blue (MB) on human dental plaque microorganisms in planktonic phase and in biofilms. Dental plaque samples were obtained from 10 subjects with chronic periodontitis. Suspensions of plaque microorganisms from 5 subjects were sensitized with MB (25 μg/ml) for 5 minutes followed by exposure to red light. Multi-species microbial biofilms developed from the same plaque samples were also exposed to MB (25 μg/ml) and the same light conditions as their planktonic counterparts. In a second set of experiments, biofilms were developed with plaque bacteria from 5 subjects and sensitized with 25 and 50 μg/ml MB followed by exposure to light as above. After PDT, survival fractions were calculated from colony-forming unit counts. In suspension, PDT produced approximately 63% killing of bacteria. In biofilms, the effect of PDT resulted in much lower reductions of microorganisms (32% maximal killing). Oral bacteria in biofilms are less affected by PDT than bacteria in planktonic phase. The antibacterial effect of PDT is reduced in biofilm bacteria but not to the same degree as has been reported for treatment with antibiotics under similar conditions.
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影响因子：
6.7
作者：
Feres, M;Haffajee, AD;Socransky, SS
通讯作者：
Socransky, SS
影响因子：
7.6
作者：
DEJONG, MH;VANDERHOEVEN, JS;VANOS, JH
通讯作者：
VANOS, JH
影响因子：
2.7
作者：
Foley, I;Gilbert, P
通讯作者：
Gilbert, P
影响因子：
2.1
作者：
Qin, Yanli;Luan, Xiuling;Zhang, Zhiguo
通讯作者：
Zhang, Zhiguo
影响因子：
3.5
作者：
SARKAR, S;WILSON, M
通讯作者：
WILSON, M