Control of Arabidopsis lateral root primordium boundaries by MYB36.
Control of Arabidopsis lateral root primordium boundaries by MYB36.
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DOI：
10.1111/nph.14304
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Gutierrez C
中科院分区：
文献类型：
--
作者：
Fernández-Marcos M;Desvoyes B;Manzano C;Liberman LM;Benfey PN;Del Pozo JC;Gutierrez C
植物的根分枝依赖于侧根的从头形成。这些细胞由创始细胞启动，触发新的形成性分裂，产生侧根原基(LRP)。LRP的大小和形状取决于控制细胞增殖的正负信号之间的平衡。目前对LRP细胞增殖潜能的调控机制尚不清楚。我们发现，拟南芥MYB36在控制LRP发育的后期阶段发挥着新的作用。拟南芥MYB36以前被证明调控初级根中Casparian条带形成和从增殖到分化的转换所需的基因。我们发现MYB36是LR发育后期的一个新的成分。MYB36在LRP周围的细胞中表达，在那里它控制着一组维持ROS平衡的过氧化物酶基因。这是确定LRP内增殖细胞和停滞细胞之间的过渡所必需的，这与从扁平到穹顶形状的原基的变化相一致。降低myb36-5中过氧化氢(H_2O_2)的水平可以显著挽救突变表型。我们的结果揭示了MYB36在LRP发育过程中内胚层外的作用，其机制类似于调节根分生组织中的增殖/分化转变。
Root branching in plants relies on the de novo formation of lateral roots (LRs). These are initiated from founder cells, triggering new formative divisions that generate lateral root primordia (LRP). The LRP size and shape depends on the balance between positive and negative signals that control cell proliferation. The mechanisms controlling proliferation potential of LRP cells remains poorly understood. We found that Arabidopsis thaliana MYB36, which have been previously shown to regulate genes required for Casparian strip formation and the transition from proliferation to differentiation in the primary root, plays a new role in controlling LRP development at later stages. We found that MYB36 is a novel component of LR development at later stages. MYB36 was expressed in the cells surrounding LRP where it controls a set of peroxidase genes, which maintain ROS balance. This was required to define the transition between proliferating and arrested cells inside the LRP, coinciding with the change from flat to dome-shaped primordia. Reducing the levels of hydrogen peroxide (H2O2) in myb36-5 significantly rescues the mutant phenotype. Our results uncover a role for MYB36 outside the endodermis during LRP development through a mechanism analogous to regulating the proliferation/differentiation transition in the root meristem.
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