Bone defect animal models for testing efficacy of bone substitute biomaterials.
Bone defect animal models for testing efficacy of bone substitute biomaterials.
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DOI：
10.1016/j.jot.2015.05.002
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发表时间：
2015-07
影响因子：
6.6
通讯作者：
Lai YX
中科院分区：
文献类型：
--
作者：
Li Y;Chen SK;Li L;Qin L;Wang XL;Lai YX
大面积骨缺损是严重的并发症，最常见的原因是广泛的创伤、肿瘤、感染或先天性肌肉骨骼疾病。如果发生骨不连，植入修复骨缺损的生物材料作为缺损填充物，可以促进骨再生，是必不可少的。为了评价生物材料作为骨缺损修复的骨替代材料，有必要建立临床相关的体外和体内测试模型，以研究其生物相容性，力学性能，降解以及与培养基或宿主组织的相互作用。体外实验的结果显著有助于评价细胞对替代生物材料的直接反应，体内试验是体外试验和人体临床试验之间的中间步骤。因此，开发或采用合适的体内骨缺损动物模型来测试骨替代物修复缺损是非常必要的。本文介绍和讨论了目前最常用的骨缺损动物模型，以测试特定的替代生物材料。此外，我们回顾了建立相关的临床前骨缺损模型的手术方案与各种动物物种和骨替代生物材料植入后的骨再生过程的评价方法。本文为临床前研究提供了重要参考。
Large bone defects are serious complications that are most commonly caused by extensive trauma, tumour, infection, or congenital musculoskeletal disorders. If nonunion occurs, implantation for repairing bone defects with biomaterials developed as a defect filler, which can promote bone regeneration, is essential. In order to evaluate biomaterials to be developed as bone substitutes for bone defect repair, it is essential to establish clinically relevant in vitro and in vivo testing models for investigating their biocompatibility, mechanical properties, degradation, and interactional with culture medium or host tissues. The results of the in vitro experiment contribute significantly to the evaluation of direct cell response to the substitute biomaterial, and the in vivo tests constitute a step midway between in vitro tests and human clinical trials. Therefore, it is essential to develop or adopt a suitable in vivo bone defect animal model for testing bone substitutes for defect repair. This review aimed at introducing and discussing the most available and commonly used bone defect animal models for testing specific substitute biomaterials. Additionally, we reviewed surgical protocols for establishing relevant preclinical bone defect models with various animal species and the evaluation methodologies of the bone regeneration process after the implantation of bone substitute biomaterials. This review provides an important reference for preclinical studies in translational orthopaedics.
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影响因子：
2.1
作者：
Castañeda, S;Largo, R;Herrero-Beaumont, G
通讯作者：
Herrero-Beaumont, G
DOI：
10.2106/00004623-199701000-00002
发表时间：
1997-01-01
影响因子：
5.3
作者：
Ghazavi, MT;Stockley, I;Gross, AE
通讯作者：
Gross, AE
影响因子：
4.8
作者：
Aerssens, J;Boonen, S;Dequeker, J
通讯作者：
Dequeker, J
影响因子：
3.5
作者：
Bateman, J. P.;Safadi, F. F.;Wikesjoe, U. M. E.
通讯作者：
Wikesjoe, U. M. E.
影响因子：
9.7
作者：
Chen, S. -H.;Lei, M.;Qin, L.
通讯作者：
Qin, L.