Validation of a novel diagnostic standard in HPV-positive oropharyngeal squamous cell carcinoma.
Validation of a novel diagnostic standard in HPV-positive oropharyngeal squamous cell carcinoma.
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DOI：
10.1038/bjc.2013.63
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发表时间：
2013-04-02
影响因子：
8.8
通讯作者：
Robinson, M.
中科院分区：
文献类型：
--
作者：
Schache, A. G.;Liloglou, T.;Risk, J. M.;Jones, T. M.;Ma, X-J;Wang, H.;Bui, S.;Luo, Y.;Sloan, P.;Shaw, R. J.;Robinson, M.
人乳头瘤病毒（HPV）检测口咽鳞状细胞癌（OPSCC），现在提倡。在新鲜肿瘤组织中证明转录活性的高危HPV（HR-HPV）被认为是分析的“金标准”。临床试验主要集中在福尔马林固定石蜡包埋（FFPE）组织上，但牺牲了灵敏度和特异性。最近，一种新的RNA原位杂交试验（RNAscope）已被开发用于检测HR-HPV在FFPE组织;然而，验证对“金标准”尚未报道。使用HR-HPV RNAscope测试包含来自79个OPSCC的FFPE核心的组织微阵列。通过与参考检测进行比较，确定了分析准确性和预后能力;在匹配的新鲜冷冻样本上进行HR-HPV的qRT-PCR。高危型HPV RNAscope的敏感性和特异性分别为97%和93%。Kaplan-Meier估计的RNAscope疾病特异性生存期（DSS，P=0.001）和总生存期（OS，P<0.001）与参考检测（DSS，P=0.003，OS，P<0.001）相似，至少不劣于基于p16免疫组化+/− HR-HPV DNA的检测。HR-HPV RNAscope相对于“金标准”表现出出色的分析和预后性能。这些数据表明，该测试可以开发为HPV相关OPSCC的诊断提供“临床标准”。
Human papillomavirus (HPV) testing in oropharyngeal squamous cell carcinoma (OPSCC) is now advocated. Demonstration of transcriptionally active high-risk HPV (HR-HPV) in fresh tumour tissue is considered to be the analytical ‘gold standard'. Clinical testing has focused on formalin-fixed paraffin-embedded (FFPE) tissue at the expense of sensitivity and specificity. Recently, a novel RNA in situ hybridisation test (RNAscope) has been developed for the detection of HR-HPV in FFPE tissue; however, validation against the ‘gold standard' has not been reported. A tissue microarray comprising FFPE cores from 79 OPSCC was tested using HR-HPV RNAscope. Analytical accuracy and prognostic capacity were established by comparison with the reference test; qRT–PCR for HR-HPV on matched fresh-frozen samples. High-risk HPV RNAscope had a sensitivity and specificity of 97 and 93%, respectively, against the reference test. Kaplan–Meier estimates of disease-specific survival (DSS, P=0.001) and overall survival (OS, P<0.001) by RNAscope were similar to the reference test (DSS, P=0.003, OS, P<0.001) and at least, not inferior to p16 immunohistochemistry +/− HR-HPV DNA-based tests. HR-HPV RNAscope demonstrates excellent analytical and prognostic performance against the ‘gold standard'. These data suggest that the test could be developed to provide the ‘clinical standard' for assigning a diagnosis of HPV-related OPSCC.
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DOI：
10.1158/1078-0432.ccr-11-0388
发表时间：
2011-10-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Schache AG;Liloglou T;Risk JM;Filia A;Jones TM;Sheard J;Woolgar JA;Helliwell TR;Triantafyllou A;Robinson M;Sloan P;Harvey-Woodworth C;Sisson D;Shaw RJ
通讯作者：
Shaw RJ
影响因子：
6.4
作者：
Lewis, James S., Jr.;Ukpo, Odey C.;Chernock, Rebecca D.
通讯作者：
Chernock, Rebecca D.
DOI：
10.1097/pas.0b013e318253a2d1
发表时间：
2012-07
期刊：
The American journal of surgical pathology
影响因子：
--
作者：
Jordan RC;Lingen MW;Perez-Ordonez B;He X;Pickard R;Koluder M;Jiang B;Wakely P;Xiao W;Gillison ML
通讯作者：
Gillison ML
影响因子：
6.2
作者：
Singhi, Aatur D.;Westra, William H.
通讯作者：
Westra, William H.
影响因子：
6.2
作者：
Sturgis, Erich M.;Cinciripini, Paul M.
通讯作者：
Cinciripini, Paul M.