Transplanted human intestinal organoids: a resource for modeling human intestinal development.
Transplanted human intestinal organoids: a resource for modeling human intestinal development.
复制标题
DOI：
10.1242/dev.201416
复制
发表时间：
2023-05-01
期刊：
影响因子：
--
通讯作者：
Helmrath MA
中科院分区：
文献类型：
--
作者：
Singh A;Poling HM;Chaturvedi P;Thorner K;Sundaram N;Kechele DO;Childs CJ;McCauley HA;Fisher GW;Brown NE;Spence JR;Wells JM;Helmrath MA
The in vitro differentiation of pluripotent stem cells into human intestinal organoids (HIOs) has served as a powerful means for creating complex three-dimensional intestinal structures. Owing to their diverse cell populations, transplantation into an animal host is supported with this system and allows the temporal formation of fully laminated structures, including crypt-villus architecture and smooth muscle layers that resemble native human intestine. Although the endpoint of HIO engraftment has been well described, here we aim to elucidate the developmental stages of HIO engraftment and establish whether it parallels fetal human intestinal development. We analyzed a time course of transplanted HIOs histologically at 2, 4, 6 and 8 weeks post-transplantation, and demonstrated that HIO maturation closely resembles key stages of fetal human intestinal development. We also utilized single-nuclear RNA sequencing to determine and track the emergence of distinct cell populations over time, and validated our transcriptomic data through in situ protein expression. These observations suggest that transplanted HIOs do indeed recapitulate early intestinal development, solidifying their value as a human intestinal model system. Summary: Histological and transcriptomic comparison of transplanted human intestinal organoids and human fetal intestinal tissue at different developmental stages reveals that these organoids are an effective tool for modeling human intestinal development.
登录
查看更多内容
影响因子：
11.8
作者：
Elmentaite R;Ross ADB;Roberts K;James KR;Ortmann D;Gomes T;Nayak K;Tuck L;Pritchard S;Bayraktar OA;Heuschkel R;Vallier L;Teichmann SA;Zilbauer M
通讯作者：
Zilbauer M
影响因子：
3.2
作者：
Pusztaszeri, MP;Seelentag, W;Bosman, FT
通讯作者：
Bosman, FT
影响因子：
28.1
作者：
Poling HM;Wu D;Brown N;Baker M;Hausfeld TA;Huynh N;Chaffron S;Dunn JCY;Hogan SP;Wells JM;Helmrath MA;Mahe MM
通讯作者：
Mahe MM
影响因子：
64.5
作者：
Kinchen J;Chen HH;Parikh K;Antanaviciute A;Jagielowicz M;Fawkner-Corbett D;Ashley N;Cubitt L;Mellado-Gomez E;Attar M;Sharma E;Wills Q;Bowden R;Richter FC;Ahern D;Puri KD;Henault J;Gervais F;Koohy H;Simmons A
通讯作者：
Simmons A
影响因子：
--
作者：
MOXEY, PC;TRIER, JS
通讯作者：
TRIER, JS