Tobacco OPBP1 enhances salt tolerance and disease resistance of transgenic rice.
Tobacco OPBP1 enhances salt tolerance and disease resistance of transgenic rice.
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DOI：
10.3390/ijms9122601
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发表时间：
2008-12
影响因子：
5.6
通讯作者：
Guo, Zejian
中科院分区：
文献类型：
--
作者：
Chen, Xujun;Guo, Zejian
渗调蛋白启动子结合蛋白1（OPBP1）是烟草的一种AP2/ERF转录因子，已被证明在烟草的抗病性和耐盐性中起作用。为了提高水稻的抗逆能力，我们构建了过量表达OPBP1的水稻植株。用250 mM氯化钠进行盐冲击处理表明，大多数OPBP1转基因植株能够存活，而对照幼苗则不能。用在200 mM氯化钠中生长两周的幼苗进行实验也得到了类似的恢复结果。还通过百草枯处理研究了OPBP1转基因植株和对照植株对氧化胁迫的耐受性，结果显示与对照植株相比，转基因株系受到的损伤较小。此外，OPBP1过量表达株系对稻瘟病菌和立枯丝核菌病原体的感染表现出更强的抗性。基因表达分析表明，几个与胁迫相关的基因的mRNA积累增加。这些结果表明，OPBP1基因的表达提高了水稻的解毒能力。
Osmotin promoter binding protein 1 (OPBP1), an AP2/ERF transcription factor of tobacco, has been demonstrated to function in disease resistance and salt tolerance in tobacco. To increase stress tolerant capability of rice, we generated rice plants with an OPBP1 overexpressing construct. Salinity shock treatment with 250 mM NaCl indicated that most of the OPBP1 transgenic plants can survive, whereas the control seedlings cannot. Similar recovery was found by using the seedlings grown in 200 mM NaCl for two weeks. The OPBP1 transgenic and control plants were also studied for oxidative stress tolerance by treatment with paraquat, showing the transgenic lines were damaged less in comparison with the control plants. Further, the OPBP1 overexpression lines exhibited enhanced resistance to infections of Magnaporthe oryzae and Rhizoctonia solani pathogens. Gene expressing analysis showed increase in mRNA accumulation of several stress related genes. These results suggest that expression of OPBP1 gene increase the detoxification capability of rice.
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