Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD.
Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD.
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DOI：
10.1186/s41021-018-0095-2
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Yamato H
中科院分区：
文献类型：
--
作者：
Kawai K;Kasai H;Li YS;Kawasaki Y;Watanabe S;Ohta M;Honda T;Yamato H
氧化应激会导致多种疾病。目前，尿8-羟基脱氧鸟苷(8-OHdG)作为氧化应激生物标志物被广泛测定。如果唾液可以作为样本来测量氧化应激生物标记物，这有一个特别的优势，因为唾液比尿液更容易收集。在这项研究中，我们研究了8-羟基鸟嘌呤(8-OHGua)作为一种氧化应激标志物在唾液中的测定，采用了配备电化学检测器的柱切换高效液相色谱系统(HPLC-ECD)。用高效液相色谱-电子捕获检测器可检测到唾液中的8-OHGua为单峰。在普通不吸烟的受试者中，唾液中8-OHGua的平均水平为3.80 ng/ml。吸烟者唾液中8-OHGua水平显著高于非吸烟者。唾液8-OHGua可能是一种有用的、无创的、有前景的氧化应激生物标志物。
Oxidative stress leads to many kinds of diseases. Currently, urinary 8-hydroxydeoxyguanosine (8-OHdG) is widely measured as an oxidative stress biomarker. There is a specific advantage if saliva can be used as the sample to measure the oxidative stress biomarker, because saliva is much easier to collect than urine. In this study, we investigated the measurement of 8-hydroxyguanine (8-OHGua) as an oxidative stress marker in saliva, by a column switching HPLC system equipped with an electrochemical detector (HPLC-ECD). The 8-OHGua in saliva could be detected as a single peak by HPLC-ECD. The average level of 8-OHGua in saliva was 3.80 ng/mL in ordinary, non-smoking subjects. The salivary 8-OHGua levels of smokers were significantly higher than those of non-smokers. Salivary 8-OHGua may be a useful noninvasive and promising oxidative stress biomarker.
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影响因子：
7.4
作者：
Song, Ming-Fen;Li, Yun-Shan;Ogawa, Yasutaka
通讯作者：
Ogawa, Yasutaka
影响因子：
5
作者：
Hu, Chiung-Wen;Huang, Yu-Jie;Chao, Mu-Rong
通讯作者：
Chao, Mu-Rong
影响因子：
6.6
作者：
Svoboda, Peter;Maekawa, Muneyuki;Kasai, Hiroshi
通讯作者：
Kasai, Hiroshi
DOI：
10.1186/s41021-016-0051-y
发表时间：
2017
期刊：
Genes and environment : the official journal of the Japanese Environmental Mutagen Society
影响因子：
--
作者：
Suzuki T;Kamiya H
通讯作者：
Kamiya H
影响因子：
6.1
作者：
Hu, Chiung-Wen;Cooke, Marcus S.;Chao, Mu-Rong
通讯作者：
Chao, Mu-Rong