Nuclei-specific deposits of iron and calcium in the rat thalamus after status epilepticus revealed with quantitative susceptibility mapping (QSM).
Nuclei-specific deposits of iron and calcium in the rat thalamus after status epilepticus revealed with quantitative susceptibility mapping (QSM).
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DOI：
10.1002/jmri.25777
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Sierra A
中科院分区：
文献类型：
--
作者：
Aggarwal M;Li X;Gröhn O;Sierra A
目的：应用定量易感地形图(QSM)研究匹罗卡品致痫后大鼠脑组织的病理变化。采用三维多回波梯度回波(GRE)技术，对注射匹罗卡品的大鼠和年龄匹配的对照组大鼠在11.7T时的脑组织进行了三维成像。根据获得的三维GRE幅度和相位数据分别计算R2*图和定量磁化率图。将QSM和R2*MAP与Perls‘s(铁)和茜素红-S(钙)染色比较，以探讨易感性对比的病理生理学基础。匹罗卡品处理的大鼠在丘脑可复制区域检测到双侧对称的损害，在R2*图上表现为高信号。相比之下，定量敏感性图显示病变内部的不同对比，有明显的高信号(顺磁)和低信号(抗磁)区域。与组织学评估比较，发现在丘脑核团内有铁和钙阳性颗粒的局限性沉积，分别对应于磁化率图中描绘的顺磁区和抗磁区。经背景校正的相位图和重建的磁化率图在病变中观察到明显的差异，表明在相图上不能可靠地区分铁和钙的沉积。多元线性回归分析显示，磁感值与病灶中铁和钙的光密度(OD)呈显著正相关(R2=0.42p<；0.001)，与铁的光密度呈正相关，与钙的光密度呈负相关。与R2*或GRE时相图相比，QSM能检测和鉴别病理性铁、钙沉积，具有较高的灵敏度和更高的空间精度，是诊断癫痫持续状态后丘脑病变的一种有前途的技术。
To investigate pathological changes in the rat brain after pilocarpine-induced status epilepticus using quantitative susceptibility mapping (QSM). Three-dimensional (3D) multi-echo gradient-echo (GRE) data were acquired from ex vivo brains of pilocarpine-injected and age-matched control rats at 11.7T. Maps of R2* and quantitative susceptibility were calculated from the acquired 3D GRE magnitude and phase data, respectively. QSM and R2* maps were compared with Perls’ (iron) and Alizarin-red-S (calcium) stainings in the same brains to investigate the pathophysiological basis of susceptibility contrast. Bilaterally-symmetric lesions were detected in reproducible thalamic regions of pilocarpine-treated rats, characterized by hyperintensity in R2* maps. In comparison, quantitative susceptibility maps demonstrated heterogeneous contrast within the lesions, with distinct hyperintense (paramagnetic) and hypointense (diamagnetic) areas. Comparison with histological assessment revealed localized deposits of iron- and calcium-positive granules in thalamic nuclei corresponding to paramagnetic and diamagnetic areas delineated in the susceptibility maps, respectively. Pronounced differences were observed in the lesions between background-corrected phase images and reconstructed susceptibility maps, indicating unreliable differentiation of iron and calcium deposits in phase maps. Multiple linear regression showed a significant association between susceptibility values and measured optical densities (ODs) of iron and calcium in the lesions (R2=0.42, p<0.001), with a positive dependence on OD of iron and negative dependence on OD of calcium. QSM can detect and differentiate pathological iron and calcium deposits with high sensitivity and improved spatial accuracy compared to R2* or GRE phase images, rendering it a promising technique for diagnosing thalamic lesions after status epilepticus.
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影响因子：
5.7
作者：
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