A trade off between mlo resistance to powdery mildew and increased susceptibility of barley to a newly important disease, Ramularia leaf spot.
A trade off between mlo resistance to powdery mildew and increased susceptibility of barley to a newly important disease, Ramularia leaf spot.
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DOI：
10.1093/jxb/ert452
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发表时间：
2014-03
影响因子：
6.9
通讯作者：
Brown JK
中科院分区：
文献类型：
--
作者：
McGrann GR;Stavrinides A;Russell J;Corbitt MM;Booth A;Chartrain L;Thomas WT;Brown JK
柱隔孢叶斑病是近年来在欧洲等温带地区发生的一种严重的大麦病害。本研究探讨了mlo突变等位基因赋予的对白粉病的强抗性和对RLS的敏感性增加之间的权衡。在田间试验和苗期试验中，mlo等位基因的存在增加了RLS的严重程度。双单倍体群体的遗传分析确定了一个数量性状位点的RLS的易感性，共定位与mlo-11等位基因的抗霉菌性。mlo-11对RLS严重程度的影响是环境敏感的。不同的mlo突变在不同的遗传背景的近等基因系的分析证实，mlo等位基因增加RLS的严重性在幼苗和成年植物。为了使对霉菌的mlo抗性完全有效，需要基因ROR 1和ROR 2。RLS症状显着减少，但真菌DNA水平保持在mlo-5单突变体，这意味着ror等位基因修改真菌从内生到坏死营养的过渡高的mlo-5误差双突变体。这些结果表明，广泛使用mlo抗性来控制霉菌可能无意中刺激了RLS作为大麦主要疾病的出现。
Ramularia leaf spot (RLS), caused by the fungus Ramularia collo-cygni, is a serious, recently emerged disease of barley in Europe and other temperate regions. This study investigated the trade off between strong resistance to powdery mildew conferred by mlo mutant alleles and increased susceptibility to RLS. In field trials and seedling tests, the presence of mlo alleles increased severity of RLS. Genetic analysis of a doubled-haploid population identified one quantitative trait locus for susceptibility to RLS, colocalizing with the mlo-11 allele for mildew resistance. The effect of mlo-11 on RLS severity was environmentally sensitive. Analysis of near-isogenic lines of different mlo mutations in various genetic backgrounds confirmed that mlo alleles increased RLS severity in seedlings and adult plants. For mlo resistance to mildew to be fully effective, the genes ROR1 and ROR2 are required. RLS symptoms were significantly reduced on mlo-5 ror double mutants but fungal DNA levels remained as high as in mlo-5 single mutants, implying that ror alleles modify the transition of the fungus from endophytism to necrotrophy. These results indicate that the widespread use of mlo resistance to control mildew may have inadvertently stimulated the emergence of RLS as a major disease of barley.
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