Neuroinflammatory In Vitro Cell Culture Models and the Potential Applications for Neurological Disorders.
Neuroinflammatory In Vitro Cell Culture Models and the Potential Applications for Neurological Disorders.
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DOI：
10.3389/fphar.2021.671734
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发表时间：
2021
影响因子：
5.6
通讯作者：
Chen N
中科院分区：
文献类型：
--
作者：
Peng Y;Chu S;Yang Y;Zhang Z;Pang Z;Chen N
细胞培养物用于制药、医学和生物科学。由于体内模型的伦理和成本限制，与生物体特性更密切相关、前景广阔、可用于高通量药物筛选的可替代细胞模型刻不容缓。神经元和胶质细胞模型已广泛应用于神经系统疾病的研究。目前对神经炎症的研究导致血脑屏障（BBB）损伤。在这篇综述中，我们描述了健康和发炎的 BBB 的特征，并总结了中枢神经系统的主要永生化细胞系（PC12、SH-SY5Y、BV2、HA 和 HBMEC 等）及其在神经系统疾病的抗炎潜力中的用途。特别是，本综述讨论了与 2D 和 3D 模型中的免疫细胞相关的不同神经炎症共培养模型。综上所述，二维共培养易于实施且经济，但不能完全重现体内微环境。而被称为器官芯片或生物芯片的 3D 模型是最新且非常有前途的方法，它通过生物工程和生物技术的改进而成为可能，并且更准确地模拟 BBB 微环境。
Cell cultures are used in pharmaceutical, medical and biological sciences. Due to the ethical and cost limitations of in vivo models, the replaceable cell model that is more closely related to the characteristics of organisms, which has broad prospects and can be used for high-throughput drug screening is urgent. Neuronal and glial cell models have been widely used in the researches of neurological disorders. And the current researches on neuroinflammation contributes to blood-brain barrier (BBB) damage. In this review, we describe the features of healthy and inflamed BBB and summarize the main immortalized cell lines of the central nervous system (PC12, SH-SY5Y, BV2, HA, and HBMEC et al.) and their use in the anti-inflammatory potential of neurological disorders. Especially, different co-culture models of neuroinflammatory, in association with immune cells in both 2D and 3D models are discussed in this review. In summary, 2D co-culture is easily practicable and economical but cannot fully reproduce the microenvironment in vivo. While 3D models called organs-on-chips or biochips are the most recent and very promising approach, which made possible by bioengineering and biotechnological improvements and more accurately mimic the BBB microenvironment.
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影响因子：
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Blurton-Jones M